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Dionex VWD Operator's Manual
Solvent
Tetrahydrofuran
Tetrapropylammonium hydroxide
Toluene
Triethylamine
Water
Table D-1. Properties of Common Mobile Phases (Continued)
D.2 UV Absorbance Wavelengths of Chromophores
Table D-2
lists the wavelengths for the absorption maxima of various
chromophores.
Functional Group
Aldehyde
Amine
Anthracene
Azido
Azo
Benzene
Bromide
Carboxyl
Diphenyl
Disulfide
Esters
Ether
Iodide
Isoquinoline
Table D-2. UV Absorbance Wavelengths of Various Chromophores
110
UV Cutoff
(nm)
212
195
285
Chromophore
–CHO
–NH2
>C=N–
–N=N–
–Br
–COOH
–S–S–
–COOR
–O–
–I
Refractive
Selectivity
Index
Group
1.405
III
1.494
VII
1.398
I
1.333
VIII
Wavelength (nm)
280–300
195
252
375
190
285–400
202
255
208
200–210
246
194
255
205
185
260
218
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