Thermo Scientific Dionex Easion Operator's Manual
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Thermo Scientific Dionex Easion Operator's Manual

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Dionex Easion Ion Chromatography
System Operator's Manual
Document No. 155028
Revision 01
August 2020

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Summary of Contents for Thermo Scientific Dionex Easion

  • Page 1 Dionex Easion Ion Chromatography System Operator’s Manual Document No. 155028 Revision 01 August 2020...
  • Page 2 © 2020 by Thermo Fisher Scientific Inc. All rights reserved. Chromeleon and OnGuard are registered trademarks of Thermo Fisher Scientific Inc. in the United States. Adobe, Acrobat, and Adobe Reader are registered trademarks of Adobe Systems, Incorporated in the United States and other countries. Microsoft and Windows are registered trademarks of Microsoft Corporation in the United States and other countries.

  • Page 3: Table Of Contents

    ..........1 Overview of the Dionex Easion ....... 1 User Documentation .
  • Page 4 Operation Overview ........27 Turning On the Dionex Easion Power ......28 Connecting to Chromeleon .
  • Page 5 Contents 3.10.2 Pretreating ........36 3.10.3 Diluting .
  • Page 6 Dionex Easion Operator’s Manual 4.12 High Detector Output ........57 4.13 Baseline Noise or Drift .
  • Page 7 Contents Physical ..........85 Environmental .
  • Page 8 Dionex Easion Operator’s Manual How do I shut off the system? .......100 How do I store columns? .

  • Page 9: Introduction

    For communication between the Dionex Easion and the PC on which Chromeleon is installed, the Dionex Easion must be connected to a USB (Universal Serial Bus) port on either the PC or an external USB hub. For more information, refer to Thermo Scientific Dionex Easion Ion Chromatography System Installation Instructions (Document No.

  • Page 10: User Documentation

    Dionex Easion Operator’s Manual User Documentation 1.2.1 About This Manual The electronic version (the PDF file) of the Dionex Easion operator’s manual contains numerous hypertext links that can take you to other locations within the file. These links include: •...

  • Page 11: Related Documentation

    Thermo Scientific Dionex AS-DV Autosampler Operator’s Manual (Document No. 065259) • Manuals for columns and other consumable products, including: Thermo Scientific Dionex CRS 500 Product Manual (Document No. 031727) Thermo Scientific Dionex OnGuard II Cartridges Product Manual (Document No. 031688) Thermo Scientific Dionex Guardcap Product Manual (Document No.

  • Page 12: Safety Information

    People’s Republic of China The Dionex Easion is designed for IC (ion chromatography) applications and should not be used for any other purpose. Operation of a Dionex Easion in a manner not specified by Thermo Fisher Scientific may result in personal injury.
  • Page 13 1 • Introduction Messages d'avertissement en français Signale une situation de danger immédiat qui, si elle n'est pas évitée, entraînera des blessures graves à mortelles. Signale une situation de danger potentiel qui, si elle n'est pas évitée, pourrait entraîner des blessures graves à mortelles. Signale une situation de danger potentiel qui, si elle n'est pas évitée, pourrait entraîner des blessures mineures à...

  • Page 14: Safety Symbols

    Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations. When the Dionex Easion is delivered to you, it meets all pertinent international electromagnetic compatibility (EMC), safety, and material compliance requirements and directives.

  • Page 15: Notice On Lifting And Handling Of Thermo Scientific

    1 • Introduction 1.4.1 Notice on Lifting and Handling of Thermo Scientific Instruments For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/or bulky for one person alone to handle safely.
  • Page 16 Dionex Easion Operator’s Manual Conformité DEEE Ce produit est conforme avec la directive européenne (2012/19/EU) des Déchets d'Equipements Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant: Ce symbole indique que l'équipement ne doit pas être jeté avec les déchets ordinaires, mais doit être collecté...

  • Page 17: Description

    2 • Description Operating Features 2.1.1 Front Door and Top Cover Figure 2-1 illustrates the front door and top cover of the Dionex Easion. Regenerant Bottle Eluent Bottle Status LEDs Injection Port Figure 2-1. Dionex Easion Doc. 155028-01 08/20...
  • Page 18 Dionex Easion Operator’s Manual LEDs Three status LEDs (described below) are on the Dionex Easion front door. LED Label If On (Green) If Flashing Power Dionex Easion power is on Does not flash Ready System check passed, but System check failed (occurs if...

  • Page 19: Component Mounting Panel

    Regenerant renews the ability of the suppressor to suppress eluent conductivity. A Dionex Easion configured for anion analyses uses dilute sulfuric acid regenerant. A Dionex Easion configured for cation analyses uses tetrabutylammonium hydroxide (TBAOH) regenerant. For more information about suppressor regeneration, see Section 2.3.5.
  • Page 20 Section 2.3.2. Pump Heads The Dionex Easion includes a dual-piston serial pump. The flow rate can be set from 0.01 mL/min to 5.00 mL/min. However, for optimum performance, set the flow rate to between 0.20 and 3.00 mL/min. Setting the flow rate to 0.00 mL/min turns off the pump. For more information...

  • Page 21: Rear Panel

    The USB connector connects the Dionex Easion to the PC on which Chromeleon software is installed. For the standard system configuration of one Dionex Easion connected to the PC, connect a USB cable between the USB connector and a USB port on the PC. For more information, refer to the Dionex Easion installation instructions.
  • Page 22 Dionex Easion and the PC on which Chromeleon is installed. LED Status Description Although the Dionex Easion and the PC are linked, no data is currently being transmitted or received. Flashing The Dionex Easion and the PC are linked and data is being transmitted.
  • Page 23 2 • Description Plumbing and Waste Lines The following lines exit the Dionex Easion through the tubing chase in the lower-left corner of the rear panel: • Eluent • Regenerant • Cell outlet • Waste (sample, regenerant, and pump priming)

  • Page 24: Fluid Schematic

    Dionex Easion Operator’s Manual Fluid Schematic Figure 2-4 shows the flow path through the Dionex Easion. Figure 2-4. Dionex Easion Flow Schematic Doc. 155028-01 08/20...

  • Page 25: System Component Details

    System Component Details 2.3.1 Pump The Dionex Easion pump is a microprocessor-based isocratic eluent delivery system. Its variable speed, dual-piston series design ensures pulse-free pumping for the most demanding applications. Primary Pump Head...

  • Page 26: Pressure Transducer

    Pressure limits are selected in the Chromeleon Instrument Configuration Manager (in the Dionex Easion Properties dialog box). For troubleshooting guidance if a pressure limit is exceeded, see Section 4.7.

  • Page 27: Injection Valve With Sample Loop

    2 • Description Pulse Damper Flow output from the pressure transducer continues to the pulse damper, which smooths minor pressure variations. From there, flow is directed to the injection valve and then to the remainder of the chromatography system. 2.3.3 Injection Valve with Sample Loop The injection valve is a six-port, electrically-activated Rheodyne valve.

  • Page 28: Dionex Crs 500 Suppressor

    Dionex Easion Operator’s Manual column, carrying the contents of the sample loop with it. For a description of how to inject samples manually, see Section 3.11.2. For a description of how to inject samples with an autosampler, see Section 3.11.4.
  • Page 29 2 • Description Figure 2-7. Anion Displacement Chemical Regeneration (DCR) Figure 2-8. Cation Displacement Chemical Regeneration (DCR) Doc. 155028-01 08/20...

  • Page 30: Conductivity Cell And Dionex Ds5 Detection Stabilizer

    Temperature compensation further improves baseline stability. Direct conductive heating is used in the Dionex Easion conductivity cell to provide temperature control and compensation. A heat exchanger inside the cell regulates the temperature. All data is collected at 40 °C (104 °F).

  • Page 31: Chromeleon Software

    Chromeleon Help. Chromeleon Software The Chromeleon 7 Chromatography Data System (version 7.2.10 MUa and later) is used to control the Dionex Easion. Chromeleon provides complete instrument control, data acquisition, and data management. 2.4.1 The Chromeleon ePanel Set The Chromeleon ePanel Set provides access to detailed status and control functions for each system component (pump, detector, and so on).

  • Page 32: Software Control Modes

    Figure 2-10 shows ePanel features related to the Dionex Easion. Figure 2-10. Dionex Easion: Chromeleon ePanel Status and Control Functions 2.4.2 Software Control Modes Two modes of software control are available: direct control and programmed control.

  • Page 33: System Wellness

    2 • Description 2.4.3 System Wellness System Wellness monitors the overall “health” of a chromatographic system. It provides built-in diagnostic and calibration features that help prevent unscheduled system shutdowns and assure reliable operation of system devices. For more information, see Section 5.1.
  • Page 34 Dionex Easion Operator’s Manual Doc. 155028-01 08/20...

  • Page 35: Operation And Maintenance

    3 • Operation and Maintenance This chapter describes routine operating and maintenance procedures for the Dionex Easion. Operation Overview Figure 3-1 illustrates the basic steps for routine operation of the Dionex Easion. Turn on the Dionex Easion power Connect to Chromeleon...

  • Page 36: Turning On The Dionex Easion Power

    • The conductivity detector begins reading the current conductivity. Connecting to Chromeleon If you are beginning operation of a Dionex Easion that has not been configured in a Chromeleon instrument, refer to the Chromeleon Help for setup instructions before proceeding.

  • Page 37: Preparing The Regenerant

    3 • Operation and Maintenance Preparing the Regenerant The type of regenerant used with the Dionex Easion depends on the analysis. A dilute sulfuric acid regenerant is used for anion analyses; a tetrabutylammonium hydroxide (TBAOH) regenerant is used for cation analyses. Follow the instructions below to prepare either regenerant.
  • Page 38 6. Fill the bottle almost to the top with deionized water, and then place the bottle in the tray on the top of the Dionex Easion. NOTE To avoid staining the Dionex Easion, be careful not to spill TBAOH on the system.

  • Page 39: Priming The Pump

    3 • Operation and Maintenance ELUENT Bottle Out REGEN REGEN Bottle Out Bottle In Figure 3-2. Eluent and Regenerant Bottle Liquid Line Connections Priming the Pump Prime the pump if the eluent has been changed, the eluent line contains air, or the pump heads are dry (for example, after servicing).
  • Page 40 Prime option. 5. The pump will begin pumping at about 3 mL/min. Continue priming the Dionex Easion until no air bubbles are exiting the pump waste line. 6. Click the pump Off button on the Chromeleon ePanel.
  • Page 41 3 • Operation and Maintenance Priming the Eluent Line with a Syringe (Optional) A syringe can be used to facilitate priming when the eluent line is empty or the pump heads are completely dry. 1. Verify that the pump is turned off. 2.

  • Page 42: Equilibrating The System

    Note that column manuals typically provide pressure specifications for systems that do not include a guard column. If a guard column is installed in the Dionex Easion, the system pressure will be 15% to 20% higher than the specification in the column manual.

  • Page 43: Verifying Operational Status

    3.6). Configuring Standby Mode The Dionex Easion can be configured to enter standby mode after a period of inactivity (defined as a period of time in which no data collection and no input from Chromeleon has occurred). In standby mode, the pump flow rate is reduced.

  • Page 44: Pretreating

    Dionex Easion Operator’s Manual • Analyze samples containing nitrite or sulfite as soon as possible. Nitrite oxidizes to nitrate, and sulfite to sulfate, thus increasing the measured concentrations of these ions in the sample. In general, samples that do not contain nitrite or sulfite can be refrigerated for at least one week with no significant changes in anion concentrations.
  • Page 45 3 • Operation and Maintenance diluting with eluent minimizes the effect of the water dip at the beginning of the chromatogram. If you dilute the sample with eluent, use eluent from the same lot to prepare the calibration standards, also. This is most important for fluoride and chloride, which elute near the water dip.

  • Page 46: Processing Samples

    Dionex Easion Operator’s Manual 3.11 Processing Samples 3.11.1 Overview Samples can be run manually (one at a time) or grouped and run automatically (in batches). Figure 3-5 shows the typical steps for each type of sample processing. Manual Automated Sample Processing...

  • Page 47: Manual Sample Processing

    1. Complete the instructions in Section 3.2 through Section 3.10 prepare the Dionex Easion for operation and to prepare the sample for processing. 2. Load the sample into the injection valve sample loop with either the Dionex AS-DV (see Section 3.11.4) or a syringe (see...

  • Page 48: Automated (Batch) Sample Processing

    Section 3.2 through Section 3.10 prepare the Dionex Easion for operation and to prepare the samples for processing. 2. If a Dionex AS-DV is installed: Prepare and fill sample vials and place them in the autosampler tray. Refer to the autosampler manual for detailed instructions.

  • Page 49: Loading And Injecting Samples With An Autosampler

    Manually (with a Dionex AS-DV): Click Inject on the Chromeleon ePanel. Autosampler Setup Notes Follow these steps to verify that the Dionex Easion injection valve is controlled by the correct device. 1. Open the Chromeleon Instrument Configuration Manager. 2. In the Dionex Easion Properties dialog box, click the Inject Valve tab.

  • Page 50: Loading And Injecting Samples With A Syringe

    Section 3.11.4. Loading Samples with a Syringe (Push Method) 1. Verify that the sample loading port on the front of the Dionex Easion is connected to sample port on the injection valve (see S (5) Figure 3-6).
  • Page 51 Section 3.11.3. Loading Samples with a Vacuum Syringe (Pull Method) 1. Verify that the sample loading port on the front of the Dionex Easion is connected to sample port on the injection valve (see S (5) Figure 3-6).

  • Page 52: Example Chromeleon Commands For Loading And

    Load on the Chromeleon ePanel (see Figure 2-10). 5. Insert a syringe into the sample loading port on the Dionex Easion front door and pull out the plunger to draw the sample into the injection valve. Draw at least five times the sample loop volume.

  • Page 53: Maintenance

    3 • Operation and Maintenance 3.12 Maintenance This section describes routine maintenance procedures for the Dionex Easion that users can perform. All other maintenance procedures must be performed by Thermo Fisher Scientific personnel. As Needed • Make fresh eluent. •...
  • Page 54 Dionex Easion Operator’s Manual Dionex Easion Preventive Maintenance Kit (P/N 088138) is available for this purpose. The kit provides parts and instructions for performing the procedure. • If a Dionex AS-DV is installed, perform the recommended annual preventive maintenance procedure. A Dionex AS-DV Preventive Maintenance Kit (P/N 072678) is available for this purpose.

  • Page 55: Troubleshooting

    4 • Troubleshooting This chapter is a guide to troubleshooting problems that may occur while operating the Dionex Easion. • For descriptions of error messages that may be displayed in the Chromeleon audit trail and suggestions for how to troubleshoot them, see Section 4.1.
  • Page 56 Abort page 51 Conductivity exceeds limit. If this error occurs when you turn on the Dionex Easion power, you can disregard it. If the error occurs at other times, it should be resolved. To troubleshoot: Refer to the suppressor manual for troubleshooting procedures.
  • Page 57 1. If a sequence is being executed, click Stop on the Chromeleon ePanel to cancel the current injection and stop the sequence. 2. Turn off the Dionex Easion power briefly and then restart. 3. Try to toggle the valve from Load to Inject by clicking Inject on the Chromeleon ePanel.
  • Page 58 For the Eluent Fill Volume to be accurate, you must enter the level each time the bottle is filled. The Dionex Easion does not automatically detect when the bottle is filled, nor when it is emptied. Doc. 155028-01 08/20...
  • Page 59 4 • Troubleshooting The system pressure is below the low pressure limit. If the system pressure falls below the minimum pressure limit for 0.5 second, Chromeleon stops the pump and this error message is displayed. The minimum pressure limit can be set to between 0 and 33.7 MPa (0 and 4900 psi) in the Chromeleon Instrument Configuration Manager.

  • Page 60: Liquid Leaks

    Check that the lines are not crimped or otherwise blocked. Also, if the blocked line is a waste line, make sure it is not elevated at any point after it exits the Dionex Easion. If a line is blocked, replace it (see Section 5.2).

  • Page 61: Pump Difficult To Prime Or Loses Prime

    4 • Troubleshooting • Leaking Dionex CRS 500 Refer to the suppressor manual for troubleshooting procedures. • Leaking injection valve 1. Make sure the liquid line connections to the valve are tight. Replace any damaged fittings. Refer to Installation of Dionex Liquid Line Fittings (Document No.

  • Page 62: No Flow

    Dionex Easion Operator’s Manual • No communication between Dionex Easion and Chromeleon ( Link on rear panel fails to light) The USB cable is not connected correctly. For connection instructions, refer to the Dionex Easion installation instructions. No Flow •...

  • Page 63: Peak "Ghosting

    4 • Troubleshooting • Flow rate through columns too high Check the pump flow rate. • Clogged column bed supports Refer to the column manual for troubleshooting procedures. • Contaminated columns Clean the columns as instructed in the column manual. •...

  • Page 64: Nonreproducible Peak Height Or Retention Time

    Dionex Easion Operator’s Manual Nonreproducible Peak Height or Retention Time • Column overloading Dilute the sample (see Section 3.10.3). • Liquid leaks Locate and eliminate the leaks (see Section 4.2). • Incomplete or imprecise filling of the sample loop 1. Fill the sample loop until excess sample exits the waste line.

  • Page 65: High Detector Output

    Chromeleon Help.) If the conductivity reading with the dummy cell is outside the tolerance range, the electronics are malfunctioning. Contact Technical Support for Dionex products for assistance. The Dionex Easion electronics components cannot be serviced by the user. 4.12 High Detector Output •...
  • Page 66 If the ambient temperature does not meet the specification of 10 to 35 °C (50 to 95 °F), verify that air conditioning and heating vents are directed away from the Dionex Easion and that the Dionex Easion front door is closed. •...
  • Page 67 • DCR waste backpressure tubing not installed (or installed incorrectly) Verify that the DCR waste backpressure tubing is installed on the end of the suppressor waste line. For more information, refer to the Dionex Easion installation instructions. Doc. 155028-01 08/20...
  • Page 68 Dionex Easion Operator’s Manual Doc. 155028-01 08/20...

  • Page 69: Service

    5 • Service This chapter describes Dionex Easion service and repair procedures that users can perform. Procedures not included here, including electronics-related repair procedures, must be performed by Thermo Fisher Scientific personnel. For assistance, contact Technical Support for Dionex products before proceeding: •...

  • Page 70: Wellness Panel Features

    Click the pump On or Off button to turn the pump on or off, respectively. • Click the Log Serial button to record the Dionex Easion serial number in the Chromeleon audit trail. • The Calibration or Diagnostic box is green while a calibration or diagnostic procedure is running.

  • Page 71: Calibrating The Pressure Transducer

    Audit Trail The Chromeleon audit trail displays an account of every event (including errors, status messages, and operational events) that occurs during Dionex Easion operation. 5.1.2 Calibrating the Pressure Transducer 1. To remove any air in the injection valve loop, toggle the injection valve position a few times by clicking Load and Inject on the Chromeleon ePanel.(You should hear a click when the valve position...
  • Page 72 Pressure Transducer Waste Valve Figure 5-1. Dionex Easion Interior Components 6. Close the waste valve. 7. Under Pressure Transducer, click Calibrate Slope. A panel with instructions and command buttons appears. 8. Follow the instructions on the panel to complete the transducer slope calibration procedure.

  • Page 73: Calibrating The Flow Rate

    5 • Service 5.1.3 Calibrating the Flow Rate Calibrate the flow rate if the Operational Qualification or Performance Qualification flow rate accuracy and precision test fails. Items needed: • 0.076-mm (0.003-in) ID yellow PEEK tubing (P/N 049715) to create 14 ± 2 MPa (2000 ± 300 psi) of backpressure (if needed) •...

  • Page 74: Replacing Tubing And Fittings

    Dionex Easion Operator’s Manual Replacing Tubing and Fittings The Dionex Easion is plumbed with the tubing and tubing assemblies listed below. Tubing Size and Type Used for... 0.125-mm (0.005-in) ID PEEK, red Connection from pump pulse damper to (P/N 044221) pressure transducer 0.25-mm (0.010-in) ID PEEK, black...

  • Page 75: Isolating A Restriction In The Liquid Plumbing

    Isolating a Restriction in the Liquid Plumbing A restriction in the liquid plumbing will cause excessive system backpressure. 1. Begin pumping eluent through the system (including the columns). 2. Follow the Dionex Easion flow schematic (see Figure 5-2) to work backward through the system, beginning at the cell exit.
  • Page 76 Dionex Easion Operator’s Manual Figure 5-2. Dionex Easion Flow Schematic Doc. 155028-01 08/20...

  • Page 77: Cleaning Eluent Bottles

    1. Click the pump Off button on the Chromeleon ePanel. 2. Open the Dionex Easion Sample Loop front door. 3. Disconnect the sample loop from ports on the injection valve (see Figure 5-3). Figure 5-3. Dionex Easion Injection Valve Doc. 155028-01 08/20...

  • Page 78: Replacing Pump Check Valves

    (see Section 5.7). To remove the old check valves: 1. Turn off the Dionex Easion power switch. 2. Disconnect the power cord. 3. Put on standard disposable laboratory rubber gloves (powder-free, particle- free, and oil-free) before disassembling the pump head.
  • Page 79 5 • Service Outlet Check Valve Secondary Primary Pump Head Pump Head Inlet Check Valve Figure 5-4. Pump Heads To install the new check valves: 1. Inspect the new inlet check valve Inlet Check Valve Outlet Check Valve assembly (P/N 045722) to verify that the 1/4-28 Port 10-32 Port double-hole end of the cartridge is...

  • Page 80: Cleaning Pump Check Valves

    6. Prime the pump (see Section 3.6). 7. When the Dionex Easion is at operating pressure, check for leaks from the check valves. Tighten a check valve a little more only if it leaks. Cleaning Pump Check Valves If a check valve leaks or is dirty, both check valves should be replaced (see Section 5.6).

  • Page 81: Replacing A Pump Piston Seal And Backup Seal

    2. After rinsing, close the waste valve. To remove the pump head and piston: 1. Turn off the Dionex Easion power switch. 2. Disconnect the power cord. 3. Put on standard disposable laboratory rubber gloves (powder-free, particle- free, and oil-free) before disassembling the pump head.
  • Page 82 Dionex Easion Operator’s Manual Vermeiden Sie Seitwärtsbewegungen, wenn Sie den Pumpenkopg vom Kolben lösen. Andernfalls kann der Kolben brechen. 7. Place the pump head (front end down) on a clean work surface. 8. The piston does not come off as part of the pump head assembly because it is captured by a magnetic retention system.
  • Page 83 5 • Service Figure 5-7. Secondary Pump Head To install the new backup piston seal: 1. Lift the spacer off the pump head. 2. Remove the seal holder from the spacer, using one of the following methods: • With the seal holder facing up, cup the spacer Notch in your hand and blow clean laboratory air at the spacer to dislodge the seal holder.
  • Page 84 Dionex Easion Operator’s Manual 4. To install the new backup piston seal: Grooved side of backup a. Hold the new backup piston seal piston seal (P/N 063382) with the grooved side facing down, and insert the shaft of the piston through the seal.
  • Page 85 5 • Service 3. If this is the primary pump head, install a 10-32 fitting plug (P/N 042772) on the outlet check valve. Tighten the plug. If this is the secondary pump head, install a 10-32 fitting plug (P/N 042772) in both the inlet and outlet ports.
  • Page 86 Dionex Easion Operator’s Manual 5. To seat the seal, push down on the spacer until it is flush with the pump head. A clicking sound indicates that the seal is correctly seated. 6. Remove the spacer and piston and verify that the seal is flush with the surface of the pump head.

  • Page 87: Replacing A Pump Piston

    Tighten the screws evenly. To complete the procedure: 1. Reconnect all liquid lines to the pump head. 2. Close the waste valve knob. 3. Turn on the Dionex Easion power switch. 4. Prime the pump (see Section 3.6). Replacing a Pump Piston Continued leaking of the piston seal after installation of a new seal (assuming the pump head is tight) indicates a dirty, scratched, or broken piston.

  • Page 88: Replacing The Waste Valve Seal

    Dionex Easion Operator’s Manual 5.10 Replacing the Waste Valve Seal A damaged seal causes leakage around the base of the waste valve knob. 1. Turn off the Dionex Easion power switch. 2. To remove the waste valve from the pump head (see Figure...

  • Page 89: Rebuilding The Injection Valve

    1. Click the pump Off button on the Chromeleon ePanel. 2. Open the front door of the Dionex Easion. 3. Disconnect each liquid line connected to the injection valve. 4. Follow the instructions provided in the Injection Valve Rebuild Kit to replace the rotor seal, isolation seal, and stator face.
  • Page 90 CELL OUT 11. Attach the line to the port on the suppressor. ELUENT OUT ELUENT OUT 12. Close the Dionex Easion front door. 13. Reconnect the power cord and turn on the Dionex Easion power. Doc. 155028-01 08/20...

  • Page 91: Replacing The Suppressor

    Refer to the suppressor manual for guidance about when to replace a suppressor. 1. Click the pump Off button on the Chromeleon ePanel. 2. Open the front door of the Dionex Easion. 3. Disconnect the four eluent and regenerant lines from the suppressor.
  • Page 92 Dionex Easion Operator’s Manual 3. The fuse drawer is located above the power switch (see Figure 5-11). A small Fuse Drawer tab locks the fuse drawer in place. Using a small screwdriver, press the tab in and then up to release the fuse drawer.

  • Page 93: A • Specifications

    A • Specifications A.1 Electrical Main Power 100 to 240 Vac, 50 to 60 Hz, autoranging Maximum input power: 90 W Maximum line draw: 1.5 A Fuses Two fast-blow IEC 127 fuses rated 3.15 A (P/N 954745) A.2 Physical Dimensions Height without bottles: 33 cm (13 in) Height with bottles and cap tubing: 60 cm (24 in) Width: 24 cm (9.5 in)

  • Page 94: Front And Rear Panel Leds

    Front Panel LEDs Power Indicates when the power is on. Ready Indicates when the Dionex Easion is ready to acquire data; flashes if the system check fails. Indicates when the Dionex Easion is running/acquiring data; flashes if an error occurs.

  • Page 95: Injection Valve

    Dionex ACRS 500 (2 mm: <15 L Dionex CCRS 500 (4 mm): <50 L Dionex CCRS 500 (2 mm): <15 L ™ Sequential ™ With a Dionex CRS 500 and a Thermo Scientific Dionex Suppression for Carbonate Removal Device: Anions •...

  • Page 96: Conductivity Detector And Flow Cell

    Factory-calibrated before shipment to the user; calibrated in the field with memory retention and transfer Control and Provided by Chromeleon software; communication with the Data Evaluation Dionex Easion is via USB (Universal Serial Bus) Cell Body PEEK Cell Electrodes Passivated 316 stainless steel 1 L...

  • Page 97: Autosampler

    A • Specifications A.9 Autosampler Automation Dionex AS-DV Autosampler (or third-party autosampler) Using Autosampler Sequential/ Dependent on autosampler capabilities Simultaneous Injection Inline Sample Optional with Dionex CRD 200 or Dionex CRD 300 Degassing Inline Filtration Dionex AS-DV Autosampler or inline filter High Automation Conditionals using Chromeleon and post-run features Flexibility...
  • Page 98 Dionex Easion Operator’s Manual System Status Standard feature Virtual Channels Power Failure Standard feature Protection Sequential Standard feature for independent dual-system analysis Injection System Trigger Standard feature Commands and Conditionals Daily Audit Trail Standard feature Sample Audit Standard feature Trail...

  • Page 99: B • Reordering Information

    B • Reordering Information Part Number Item Pump 067701 Primary pump head assembly 067703 Secondary pump head assembly 045722 Inlet check valve assembly, 1/4-28 045721 Outlet check valve assembly, 10-32 055870 Piston seal 014895 O-ring 063382 Backup piston seal 067706 Seal holder 052840 Piston...
  • Page 100 Miscellaneous Items 052324 Microbore Tubing Kit 960777 USB cable, 1.8 m (6 ft) long 954745 IEC 127 fast-blow fuses, rated 3.15 amps Preventive Maintenance Kits 088138 Dionex Easion Preventive Maintenance Kit 072678 Dionex AS-DV Preventive Maintenance Kit Doc. 155028-01 08/20...

  • Page 101: C • Ttl And Relay Control

    C • TTL and Relay Control A 12-pin connector strip for TTL/relay control is located on the Dionex Easion rear panel. The connector provides two relay outputs, two TTL outputs, and four TTL inputs (see Figure C-1). Pin Function Description...

  • Page 102: Connecting A Ttl Or Relay

    When attaching wires to the connector plug, be careful not to allow stray strands of wire to short to the adjoining position on the connector. b. Plug the connector into the 12-pin connector on the Dionex Easion rear panel. Doc. 155028-01 08/20...

  • Page 103: Selecting Ttl Input Control Modes And Functions

    Normal Edge Thermo Scientific Dionex modules. If the device connected to the Dionex Easion does not send a normal edge signal, select the appropriate control mode. Refer to the documentation provided with the controlling device, as well as the information below, to select the correct type.
  • Page 104 Dionex Easion Operator’s Manual • Normal Edge: In normal edge Action Off or No Effect operation, the negative (falling) edge of +5 V a signal turns on the function. For NORMAL EDGE example, for the Load/Inject function, +0 V the negative edge switches the injection Action On valve position to Load.

  • Page 105: Configuring Relay Output 1 Or 2 To Respond To The Pump Flow

    1. Open the Chromeleon Instrument Configuration Manager. 2. In the Dionex Easion Properties dialog box, click the State Devices tab. 3. Select the relay output name (Pump_ECD_Relay_1 or Pump_ECD_ Relay_2) and press the F2 key.
  • Page 106 Dionex Easion Operator’s Manual 2. Expand the list of commands under Pump_ECD and select the TTL or relay output name. 3. Execute the command. To program control of TTL and relay outputs: Include commands for control of the TTL and relay outputs in the Chromeleon instrument method.

  • Page 107: D • Faq

    D • FAQ D.1 How do I connect to the Dionex AS-DV? For instructions on how to connect the Dionex Easion to the Dionex AS-DV, refer to the autosampler operator’s manual. D.2 Why are the retention times moving? Retention times can shift if the pump flow is erratic, or if the column or eluent is contaminated.

  • Page 108: When Should I Remake Standards

    Click the pump Off button on the Chromeleon ePanel (see Figure 2-10). –or– Turn off the power switch on the Dionex Easion rear panel (see Figure 2-3). D.7 How do I store columns? Columns should be stored in eluent. Refer to the column manual for complete instructions.

  • Page 109: How Do I Clean A Column

    D • FAQ D.9 How do I clean a column? Refer to the troubleshooting section of the column manual. D.10 Why is the conductivity high? Possible reasons for high conductivity include: • The suppressor needs regeneration. Refer to the troubleshooting section of the suppressor manual.
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  • Page 111: E • Introduction To Ion Chromatography (Ic)

    E • Introduction to Ion Chromatography (IC) The Dionex Easion performs isocratic ion analyses using suppressed conductivity detection. An ion chromatography system such as the Dionex Easion typically consists of a liquid eluent, a high-pressure pump, a sample injector, a separator column, a chemical suppressor, and a conductivity cell.
  • Page 112 • As the eluent and sample are pumped through the separator column, the sample ions are separated. In the Dionex Easion, the mode of separation is called ion exchange. This mode is based on the premise that different sample ions migrate through the IC column at different rates, depending upon their interactions with the ion exchange sites.

  • Page 113: F • Glossary

    F • Glossary Analytical Column Synonymous with Separator Column. Band Spreading The broadening of the sample band as it travels through the column. Band spreading can also occur in the injection valve, detector cell, and interconnecting tubing. Calibration Curve A graph showing detector response in peak height or area versus analyte concentration.
  • Page 114 Dionex Easion Operator’s Manual Channeling The preferential flow of liquid along more open, less resistant paths through the column packing. This causes Band Spreading. Column Efficiency (N) A measure of the narrowness of analyte bands as they elute from the column.
  • Page 115 F • Glossary % Crosslink Divinylbenzene content in a polystyrene/divinylbenzene (PS-DVB) resin; this contributes to the mechanical strength of the resin and determines chromatographic properties.  Equivalent Conductivity ( The contribution of an ionic species to the total conductivity of a solution as measured in a standard cell having electrodes 1 cm in area and exactly 1 cm apart.
  • Page 116 Dionex Easion Operator’s Manual Regenerant A dilute acid or base which converts ion exchange sites in the suppressor back to the form which will suppress the eluent conductivity. Resin See Ion-Exchange Resin. Resolution (R) A measure of the separation between two sample components. It is expressed as the ratio of the distance between the two peak maxima to the mean value of the peak width at the baseline.
  • Page 117 F • Glossary Void Volume (V The volume occupied by the eluent in a packed column. This volume includes the volume between the injection valve and the column, as well as between the column and the detector cell. Unretained components are eluted in the void volume.
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  • Page 119 Index Bottle Eluent, 10 Air particulate samples, 36 Regenerant, 10 Alarms, 10, 47 Bottle, eluent See also Error messages Cleaning, 69 Ambient temperature, 58 Connecting, 30 Anion eluent, 11 Monitoring the liquid level, 28 Anion regenerant, 11 Bottle, regenerant Bottle assembly, 22 Connecting, 30 Anion separations, 34 AS-DV Autosampler...
  • Page 120 Dionex Easion Operator’s Manual Communication with Easion, 14, 54 Configuring standby mode, 35 Data collection, 39 Control modes, 24 Cell temperature during, 22 Eluent Fill Volume slider, 28 Error messages, 47 See Displacement chemical regeneration Instrument methods, 40, 43 – 44...
  • Page 121 Index Top cover, 9 Requirements, 66 Tubing assemblies, 66 Flashing LED, 10 Weight, 85 Flow path, 16 Electrical specifications Flow rate, 12 Fuses, 85 Automatically reducing, 35 Main power, 85 Calibrating, 62, 65 Electrochemical detector conductivity Standby mode, 35 See Conductivity Flow rate calibration error, 48 Eluent, 11, 29 Flow rate range, 12, 86...
  • Page 122 Dionex Easion Operator’s Manual Creating instrument methods, 40 Microbore system Inverted pulse TTL input control, 96 Backpressure coil requirements, 58 Ion chromatography overview, 103 Microbore Tubing Kit, 58, 66 Ion exchange, 104 Module data buffer overflow error, 49 Isocratic delivery system, 103...
  • Page 123 Index Physical specifications Pump head waste valve Decibel level, 85 Leaking, 52 Dimensions, 85 Seal replacement, 80 Weight, 85 Pump motor lost control error, 49 Piston Pump pressure hardware error, 49 Replacing, 79 Pump pressure slope calibration error, 50 Piston seal Pump specifications Replacing, 73 –...
  • Page 124 Dionex Easion Operator’s Manual Regulatory compliance, 6 Selectivity Relay control, 93 Troubleshooting, 56 Rear panel connector, 14 Separator column, 12 Relay outputs Sequences (Chromeleon), 40 Direct control, 97 Service chase, 13 Instrument method control, 98 Service procedures, 71 Responding to pump flow, 97...
  • Page 125 Index Sulfite in samples, 36 Error messages, 47 Sulfuric acid concentrate, 29 Liquid leaks, 52 Sulfuric acid regenerant, 11 No cell response, 56 Suppressor, 12, 20 No detector response, 56 Leaking, 53 No pump flow, 54 Replacing, 83 Peak ghosting, 55 Supressor specifications Peak height is nonreproducible, 56 Chemical suppression, 87...
  • Page 126 Dionex Easion Operator’s Manual See Injection valve See Waste valve Waranty, voiding, 61 Waste lines, 15 Blocked, 52 Waste valve, 18 Opening, 18, 73 Seal replacement procedure, 80 Water samples, 36 Wellness panel (Chromeleon), 61 – 62 Audit trail, 63...

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