Product Description; Introduction; Intended Use; Chapter 2 Product Description - Thermo Scientific KingFisher Pure RNA Blood Kit Instruction Manual

Thermo Fisher Scientific

Product Description

Introduction

The KingFisher Pure RNA Blood Kit is designed for rapid automated RNA
purification from up to 200 µl of mammalian blood samples using Thermo
Scientific™ KingFisher™ instruments. See the detailed list below.
1) Fresh or frozen whole human blood samples anticoagulated by EDTA
(ethylenediaminetetraacetic acid) or citrate, and samples stabilized by
commercial stabilizers PAXgene or Tempus
2) Fresh, frozen, or stabilized (PAXgene and Tempus) blood of different
species: mouse, rabbit, canine, horse, cow, sheep, and goat
3) Human buffy coat, blood leucocytes, erythrocytes, and bone marrow samples
The RNA purified using the KingFisher Pure RNA Blood Kit is of high quality
and free of proteins, nucleases, and other contaminants or inhibitors. It is,
therefore, suitable for direct use in many different downstream applications,
such as RT-qPCR (reverse transcription quantitative PCR), RT-PCR, and
several other enzymatic reactions.

Intended Use

The KingFisher Pure RNA Blood Kit is developed for the purification of RNA from
blood samples using paramagnetic particles. The reagents and specific plastic
consumables are designed for use with the KingFisher Flex and KingFisher Duo
magnetic particle processors as part of an integrated system. The KingFisher
Pure RNA Blood Kit enables extraction of RNA from fresh or stored blood. The
KingFisher Pure RNA Blood Kit is only intended for research use, not for clinical
or diagnostic use. The user is responsible for validating the performance of the
Thermo Scientific KingFisher Pure RNA Blood Kit
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