Load Samples; Run The Gel - Thermo Scientific invitrogen E-Gel G8121ST User Manual

Load samples

1. Load prepared samples. Keep all sample
volumes uniform.
2. Load prepared DNA ladder.
Note: Total loading volume for marker lanes
in double comb E-Gel
3. Load 1X E-Gel Sample Loading Buffer or
deionized water in all empty wells.
Note: For E-Gel
E-Gel
4. Run gels within 1 minute after loading
samples.

Run the gel

1. Press Set up run to start E-Gel
2. Select the E-Gel
type.
Use the up/down arrows to navigate through the menu.
3. (Optional) For recurring experiments, select the last
used protocol.
Gel Type
E-Gel EX Agarose Gel, 1% and 2%
™
E-Gel EX Agarose Gel, 4%
™
E-Gel EX Double Comb Agarose Gels,
™
1% and 2%
E-Gel Agarose Gel with SYBR
™
1%, 2%, and 4%
E-Gel Double Comb SYBR
™
Agarose Gels, 1% and 2%
E-Gel CloneWell II Agarose Gel, 0.8%
™ ™
E-Gel SizeSelect II Agarose Gel, 2%
™ ™
E-Gel NGS Agarose Gel, 0.8%
™ ™
Reverse protocol for:
E-Gel CloneWell II Agarose Gel
™ ™
E-Gel SizeSelect II Agarose Gel
™ ™
E-Gel Power Snap Electrophoresis System User Guide
™
agarose gels is 10 μL.
™
EX agarose gels, use 0.1X
™
Sample Loading Buffer.
™
protocol selection.
™
protocol corresponding to your gel
™
Recommended program
E-Gel EX 1-2%
E-Gel EX 4%
E-Gel EX 1-2%
Safe,
™
E-Gel 0.8-2%
Safe
™
E-Gel Double Comb
CloneWell 0.8%
SizeSelect 2%
E-Gel 0.8-2%
Reverse E-Gel
Default run time Maximum run time
10 min
15 min
5 min
26 min
13 min
12 min
8 min
26 min
2 min
15 min
20 min
8 min
40 min
18 min
28 min
25 min
32 min
3 min
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