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2.1.3
Tuning and calibration information
You must tune and, if necessary, calibrate the mass spectrometer before using it. You can perform
these tasks via the IntelliStart software.
For further instruction, see the mass spectrometer's online Help topic "Instrument Setup".
2.1.4
Running the mass spectrometer at different flow rates
The ACQUITY UPLC IVD and ACQUITY UPLC I-Class IVD systems run at high flow rates. To
optimize desolvation, and thus sensitivity, run the ACQUITY Xevo TQD IVD system at appropriate
gas-flows and desolvation temperatures. When you specify a flow rate, IntelliStart software
specifies these parameter settings.
Flow rate versus temperature and gas flow:
Flow rate (mL/min) Source temp (°C)
0.000 to 0.020
0.021 to 0.100
0.101 to 0.500
>0.500
2.2
Preparing the IntelliStart fluidics system
For additional information, see
Notice:
To avoid damaging the mass spectrometer by accidently spilling solvent on it, do
not store large-volume solvent reservoirs atop it.
2.2.1
Installing the reservoir bottles
Use standard reservoir bottles (15 mL) for instrument setup and calibration. Use the Low-volume
Adaptor Kit (sold separately) to infuse smaller volumes. The low-volume vials have a volume of 1.5
mL.
2.2.1.1
Required material
Chemical-resistant, powder-free gloves
Warning:
avoid spreading contamination to uncontaminated surfaces, wear clean,
chemical-resistant, powder-free gloves while installing reservoir bottles and
low-volume vials.
Desolvation temp (°C)
150
200
150
300
150
500
150
600
Appendix D
To avoid personal contamination with biohazards or toxic materials, and to
September 7, 2016, 715003560IVD Rev. C
Page 33
Desolvation gas flow
(L/h)
800
800
1000
1000
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