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4.18.3
Cleaning the sample cone and cone gas nozzle
4.18.3.1
Required materials
•
Chemical-resistant, powder-free gloves.
•
Appropriately sized glass vessels in which to completely immerse components when
cleaning. Use only glassware not previously cleaned with surfactants.
•
HPLC-grade (or better) methanol.
•
HPLC-grade (or better) water.
•
Formic acid.
•
Ultrasonic bath.
•
Source of oil-free, inert gas (nitrogen or argon) for drying (air-drying optional).
•
Wash bottle containing HPLC-grade (or better) 1:1 methanol/water.
•
Large beaker.
Warning:
To avoid personal contamination with biohazards or toxic materials, and to avoid
spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free
gloves when working with the sample cone and cone gas nozzle.
Warning:
To avoid injury, work with extreme care. Use a fume hood, and suitable protective
equipment. Formic acid is extremely corrosive and toxic.
Notice:
To avoid damaging the fragile sample cone, do not place it on its tip; instead, place
it on its flanged base.
To clean the sample cone and cone gas nozzle:
1.
If the sample cone contains debris, place a drop of formic acid on its aperture.
2.
Immerse the sample cone, cone gas nozzle, and cone gas nozzle handle in separate glass
vessels containing 1:1 methanol/water.
Tip:
If the components are obviously contaminated, use 45:45:10 methanol/water/formic
acid.
3.
Place the vessels in the ultrasonic bath for 30 minutes.
4.
If you used formic acid in the cleaning solution, do as follows:
a.
Rinse the components by immersing them in separate glass vessels containing water
and then place the vessels in the ultrasonic bath for 20 minutes.
b.
Remove any residual water from the extraction cone by immersing it in a glass vessel
containing methanol and then place the vessel in the ultrasonic bath for 10 minutes.
Notice:
powder-free gloves for the rest of this procedure.
To avoid recontaminating the components, wear clean, chemical-resistant,
September 7, 2016, 715003560IVD Rev. C
Page 68
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