COOK Medical IVF Media Instructions For Use Manual page 8

DIRECTIONS FOR USE
• Aseptic technique should be used.
• Fertilization Medium is suitable for both open and micro culture. If using micro drops ensure
culture oil (K-SICO) is used to avoid evaporation.
• A suitable wash volume must also be prepared.
• Fertilization medium is suitable for a standard sperm exposure (16-18 hours) or a short
insemination.
• Warm the Fertilization Medium to 37°C and equilibrate in a 6% CO
of 4 hours prior to use.
• Following oocyte and sperm preparation add sperm to each well and return the dish to the
incubator until fertilization check.
• Subsequent zygotes are transferred to Cleavage Medium (K-SICM).
SYDNEY IVF PVP K SIPV 200 5
INTENDED USE
Sydney IVF PVP is intended for use as an aid in the immobilization and isolation of individual
sperm cells prior to intracytoplasmic sperm injection (ICSI) procedures.
GENERAL INFORMATION
PVP is supplemented with Human serum albumin (10 mg/mL) and gentamicin (0.01 mg/mL).
Ready to use after equilibration to 37°C and 6% CO
STORAGE AND STABILITY
PVP must be stored in original unopened container, refrigerated at 2-8°C. Do not freeze.
DIRECTIONS FOR USE
• Aseptic technique should be used.
• Warm the PVP to 37°C and equilibrate in a 6% CO
to use.
• PVP is suitable for both open and micro culture. If using micro drops ensure Culture Oil
(K-SICO) is used to avoid evaporation.
• PVP consistency can be reduced by addition of Sperm Medium (K-SISM) if required.
• For procedures that will be completed in less than 10 minutes, the injection dish should be
prepared with Cleavage Medium (K-SICM) warmed to 37°C and equilibrated in a 6% CO
incubator for a minimum of 4 hours prior to use.
• Alternately, if the procedure will take longer than 10 minutes, the injection dish should be
prepared with Gamete Buffer (K-SIGB) warmed to 37°C in air for a minimum of 4 hours prior
to use.
CLEAVAGE
SYDNEY IVF CLEAVAGE MEDIUM K SICM 20, K SICM 50 & K SICM 100
INTENDED USE
Cleavage Medium is intended for use during in vitro fertilization procedures for culture and
transfer of cleavage stage embryos.
incubator for a minimum
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incubator for a minimum of 4 hours prior
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