Product Description; Storage And Stability; Directions For Use - COOK Medical IVF Media Instructions For Use Manual

• Wash the embryo(s) before placing it in the final drop/well and returning to 37°C and 6% CO
incubator.
• The reproductive specialist may continue growth until transfer on Day 4 to 6.
SYDNEY IVF BLASTOCYST VITRIFICATION KIT K SIBV 5000
INTENDED USE
Blastocyst Vitrification Kit is intended to be used for the vitrification of Human blastocysts for
A.R.T. procedures. This kit is designed for use with Blastocyst Warming Kit (K-SIBW-5000).
GENERAL INFORMATION
Blastocyst Vitrification Kit consists of three HEPES buffered media designed for sequential use.
Vitrification solutions are supplemented with Human serum albumin (up to 20.0 mg/mL) and
gentamicin (0.01 mg/mL).
WARNING: Blastocyst Vitrification Kit is not ready for use. It requires the combination
of vitrification solutions by the end user prior to use. Vitrification Solution 4 is Dimethyl
sulphoxide (DMSO). A material Safety Data Sheet (MSDS) is available upon request.
This product must be used with a legally marketed storage device that is indicated for use in
blastocyst vitrification procedures. The storage device must be a closed storage system to
prevent the potential risk of viral contamination that exists with open storage systems.

PRODUCT DESCRIPTION

The three media are all based upon the formulation of Cryobase buffer, a 10 mM HEPES
buffered media containing 20.0 mg/mL Human Serum Albumin, 0.01 mg/mL gentamicin.
Solution 1 contains Cryobase buffer only. Solution 2 is used for preparation for vitrification
and contains Cryobase buffer with 8% ethylene glycol and 8% DMSO. Solution 3 is used during
cyrostorage and contains Cryobase buffer with 0.68 M trehalose, 16% ethylene glycol and 16%
DMSO. Solution 4 is a glass vial of DMSO.

STORAGE AND STABILITY

Blastocyst Vitrification Kit must be stored in original unopened container, refrigerated at 2-8°C.

DIRECTIONS FOR USE

Preparation
• Aseptic technique should be used.
• DMSO is a frozen solid at 2-8°C. Before use equilibrate Vitrification Solution 4 (DMSO) to
room temperature prior to use.
• Add 400 µL of Vitrification Solution 4 (DMSO) to 4.6 mL of Vitrification Solution 2 and mix well.
• Add 1 mL of Vitrification Solution 4 (DMSO) to 5.25 mL of Vitrification Solution 3 and mix well.
• Prepare the vitrification solutions in a 4 well dish by adding 800 µL of Vitrification Solution 1
into well 1 and 2, 800 µL of the prepared Vitrification Solution 2 into well 3, and 800 µL of the
prepared Vitrification Solution 3 into well 4.
• Equilibrate the three Vitrification Solutions to 37°C prior to use.
Method
• Place the embryo(s) to be cryopreserved into well 1, maximum of three embryos.
• Move the specific embryo to be vitrified into well 2. Have the allocated vitrification device
ready. Leave for a maximum of 1 minute.
• Move the embryo to well 3 for two minutes.
10
2