COOK Medical IVF Media Instructions For Use Manual page 11

• Move the embryo to well 4. Complete the next step within 20-30 seconds.
• Using a positive displacement pipette, aspirate the embryo in 1.5 μL of solution. Avoid bubbles.
• Expel the droplet containing the embryo onto vitrification device and vitrify immediately
according to laboratory approved methods.
SYDNEY IVF BLASTOCYST WARMING KIT K SIBW 5000
INTENDED USE
Blastocyst Warming Kit is intended for the warming of Human blastocysts that have undergone
vitrification using Blastocyst Vitrification Kit (K-SIBV-5000) for A.R.T. procedures.
GENERAL INFORMATION
Blastocyst Warming Kit consists of three HEPES buffered media designed for sequential use.
Warming solutions are supplemented with Human serum albumin (up to 20.0 mg mL) and
gentamicin (0.01 mg/mL).
PRODUCT DESCRIPTION
The three media are all based upon the formulation of Cryobase buffer, a 10 mM HEPES
buffered media containing 20.0 mg/mL Human Serum Albumin, 0.01 mg/mL gentamicin.
Solution 1 is the first medium used in the thawing process and contains Cryobase buffer with
0.33 M Trehalose. Solution 2 contains Cryobase buffer with 0.2 M Trehalose. Solution 3 is used
for recovery and contains Cryobase buffer only.
STORAGE AND STABILITY
Blastocyst Warming Kit must be stored in original unopened container, refrigerated at 2-8°C.
DIRECTIONS FOR USE
Preparation
• Aseptic technique should be used.
• Equilibrate the three Warming Solutions to 37°C before use.
• Prepare a suitable volume of Blastocyst Medium (K-SIBM) in an incubator with a 6% CO
environment at 37°C prior to use, for culture of the blastocyst post warming.
Method
• Identify the vitrification device(s) to be warmed and warm in accordance with the
manufacturer.
• Prepare the Warming Solutions in a 4 well dish by adding 800 µL of Warming Solution 1 into
well 1 and 2, 800 µL of Warming Solution 2 into well 3, and 800 µL of Warming Solution 3 into
well 4.
• Immediately extract the embryo from the vitrification device and place into well 1 and STIR
IMMEDIATELY until the bead dissolves.
• Using a pipette, transfer the embryo to well 2 for 5 minutes.
• Move to well 3 for 5 minutes. Wash the embryo well.
• Move to well 4 for 5 minutes. Wash the embryo well.
• Place the embryo in the dish containing equilibrated Blastocyst Medium (K-SIBM) ready for
hatching.
• If required, hatch the embryo (laser method preferred) and move to a new micro droplet of
equilibrated Blastocyst Medium (K-SIBM).
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