COOK Medical IVF Media Instructions For Use Manual page 7

• Remove the rack after 20-60 minutes and remove the medium above the semen
(approximately 0.25 mL).
• Add 5.0 mL of equilibrated Sperm Medium to the aspirated sample and centrifuge at 600 g
for 10 minutes.
• Remove the pellet and re-suspend in a small volume of equilibrated Sperm Medium or
Fertilization Medium (K-SIFM).
• Count sperm and calculate the concentration. Adjust as required. Store in a 6% CO
at 37°C until required.
FERTILIZATION
SYDNEY IVF CULTURE OIL K SICO 50 2
INTENDED USE
Culture Oil is intended to be used as an oil overlay for culture of gametes, zygotes, or embryos
in assisted reproductive technology (ART) and micro manipulation procedures.
GENERAL INFORMATION
Ready to use after equilibration to 37°C.
STORAGE AND STABILITY
Culture Oil must be stored in the original unopened container, refrigerated at 2-8°C. Do not
freeze. Protect from light.
DIRECTIONS FOR USE
• Aseptic technique should be used.
• Culture Oil is used for micro droplet culture from fertilization to the blastocyst. It can also be
used while performing ICSI, assisted hatching and embryo biopsy.
• Pipette the intended volume of medium to be used for the micro drop on the bottom of a
Petri dish.
• Gently pipette the Culture Oil into the Petri dish until the micro drops are sufficiently covered in oil.
• Equilibrate Culture Oil and bicarbonate buffered media micro drop in 6% CO
minimum of 4 hours prior to use.
• Warm Culture Oil and HEPES buffered media to 37°C before use.
SYDNEY IVF FERTILIZATION MEDIUM K SIFM 20, K SIFM 50 &
K SIFM 100
INTENDED USE
Fertilization Medium is intended for use during in vitro procedures for insemination and
incubation of oocytes.
GENERAL INFORMATION
Fertilization Medium is supplemented with Human serum albumin (5 mg/mL) and gentamicin
(0.01 mg/mL).
Ready to use after equilibration to 37°C and 6% CO
STORAGE AND STABILITY
Fertilization Medium must be stored in original unopened container, refrigerated at 2-8°C.
Do not freeze.
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incubator
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at 37°C for a
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