Tune The Lc/Ms - Agilent Technologies 6100 Series LC/MS Quick Start Manual

Tune the LC/MS

When the LC/MS is used as a detector for the LC, a mass spectrum is
associated with each data point in the LC chromatogram. To obtain high
quality, accurate mass spectra, optimize the LC/MS to:
• Maximize sensitivity
• Maintain acceptable resolution
• Ensure accurate mass assignment
Tuning is the process of adjusting LC/MS parameters to achieve these goals.
After the LC/MS parameters have been optimized, they must be saved in a
tune file (.tun). This tune file is then specified in the method that is used to
acquire data for your samples.
Frequent tuning, whether automated or manual, is not required. Once tuned,
the LC/MS is stable. Tuning is needed no more often than monthly, weekly at
most.
Wait 4 hours after pumpdown before tuning or operating your LC/MS. The
analyzer takes about 11 hours to reach thermal equilibrium. Tune files that
are created or data that is acquired before the LC/MS is at thermal
equilibrium can have incorrect mass assignments and other inaccuracies.
Task
To use Autotune
Agilent 6100 Series LC/MS System Quick Start Guide
Instructions
1 From the MSD Tune view, select
Autotune from the Tune menu or click
the Autotune toolbar button.
2 Review the tune report that is printed
automatically when tuning is
completed.
Basic Operation
Tune the LC/MS
Comments
Use autotune for automated
adjustment of the LC/MS
performance.
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