Nikon Eclipse TI-TIRF-E Setup Manual page 91

Figure 4.2-7
2
Attach the dichroic mirrors (photo activation illuminator unit only).
It is necessary to attach a dichroic mirror (optical path switch mirror), which switches between laser light and
episcopic illumination, inside the photo activation illuminator unit. Since the attachment does not ship with a
mirror attached, you must attach one following the procedure below.
You can also change the light intensity ratio of laser light and episcopic illumination by switching the dichroic
mirror and operating the optical path switch lever.
Ordinarily, you should attach a total reflection mirror. When using a total reflection mirror, operating the
optical path switch switches between 100% laser light and 100% episcopic illumination.
WARNING
To prevent hazardous laser emission from the mirror replacement opening, turn off the laser
power before replacing mirrors.
Figure 4.2-8
Chapter 4 Setting Up the Microscope
4.2 Setup Procedure
Anchoring bolt
Cap
7. Remove the anchoring bolt from the optical path
switching module.
The optical path switching module comes
equipped with an anchor for transportation. Be
sure to remove the anchor before use.
1. Unscrew the anchoring bolt from the top of
the optical path switching module.
2. To plug the hole where the anchoring bolt
was, screw in the button bolt provided.
1. Remove the retaining screw for the cap of the
mirror replacement opening using the Allen
wrench, and then remove the cap by turning it
counterclockwise.
1-77