Nikon Eclipse TI-TIRF-E Setup Manual page 134

Illuminator unit/motorized illuminator unit/photo activation illuminator unit, beamsplitter 50/50
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2
Transmitting the He-Ne2 laser beam through the optical-fiber cable
As with the He-Ne1 laser, adjust the He-Ne2 laser body position to transmit the laser beam at maximum
light intensity through the optical-fiber cable.
At this time, close the shutters for the argon and He-Ne1 laser so that only the He-Ne2 laser beam
enters the optical-fiber cable.
Incident light efficiency of about 50% should be achieved at the emitting end of the optical-fiber cable
relative to values measured in front of the coupler.
Record the light intensity.
3
Attaching the He-Ne laser to the holder
1.
While pressing the sleeve against the sleeve-mounting section, lightly tighten the slot-tightening
screw.
During this process, light intensity may decrease slightly. Work carefully, while monitoring the light
intensity at the end of the optical-fiber cable on the power meter.
2.
Tighten the front and rear sleeve-retaining screws of each laser.
4
Laser intensity measurement
At first, maximize each laser intensity. Move off all of movable ND filters from the optical path and
adjust Argon laser to its maximum power. Measure and record the light intensity of each laser emitting
from the optical fiber with a calibrated laser intensity power meter.
5
Handling of cables and codes.
Fix cables and codes with bundling band.
Chapter 5 Laser Unit
5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit
Sleeve-mounting section
Sleeve
Sleeve-retaining screw
Slot-tightening screw
Figure 5.2-30
1-120
He-Ne2
Ar

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