Nikon Eclipse TI-TIRF-E Setup Manual page 132

Illuminator unit/motorized illuminator unit/photo activation illuminator unit, beamsplitter 50/50
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5
Adjusting to give maximum Ar laser light transmission through the fiber
Fiber side
adjustment
screw
Figure 5.2-26
Figure 5.2-27
Figure 5.2-28
Chapter 5 Laser Unit
5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit
Laser incidence
side adjustment
screw
1.
Attach the emitter end of the optical fiber to the power
meter tool and adjust to find the point giving
maximum brightness, while measuring the brightness
on the power meter. To adjust brightness, use either
the laser incidence side H adjustment screw
(horizontal) or V adjustment screw (vertical), together
with the fiber side V adjustment screw and H
adjustment screw.
As a guide to Ar laser incidence efficiency, the
brightness at the end of the coupler should be at least
50% of the brightness in front of the coupler.
(Adjustment example)
(1) Turn the laser incidence side H adjustment
screw 30 to 60 degrees in one direction.
(2) Turn the fiber side H adjustment screw in the
same direction as (1) to find the point giving a
maximum brightness.
If brightness does not increase, turn the laser
incidence side adjustment screw 30 to 60
degrees in the direction opposite to (1). Turn the
fiber side adjustment screw in the same
direction to find the point giving a maximum
brightness.
(3) Repeat the adjustment in step (2) to find the
point in the horizontal axis giving a maximum
brightness.
(4) Turn the laser incidence side V adjustment
screw 30 to 60 degrees. Turn the fiber side V
adjustment screw in the same direction to find
the point giving a maximum brightness.
(5) Repeat the adjustment in step (4) to find the
point in the vertical axis giving a maximum
brightness.
(6) Repeated adjustments along the horizontal and
vertical axes may result in even greater
brightness.
2.
Record the Ar laser brightness. (Note the power
meter wavelength setting.)
3.
Open the other laser shutters and check that the
laser light is emitted. If no light is emitted from the
other lasers, repeat the adjustments from "6.
Centering the He-Ne laser" in "5.2.3 Laser Rough
Adjustment" in Part 1.
1-118

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