Nikon Eclipse TI-TIRF-E Setup Manual page 311

5.2 Installation of the LU4-B5 Beamsplitter 50/50 (TIRF-PAU system)
Figure 5.2-7
4
Adjusting the reference optical fiber
Reference fiber coupling
Figure 5.2-8
5
Adjusting the additional optical fiber
Additional fiber coupling
Figure 5.2-9
This is the end of the adjustment of the additional optical fiber.
Chapter 5 Laser Unit
Manually open the shutter with a tool.
Adjust the optical axis so that the 488-nm laser beam,
the reference for other lasers, passes through the
centering tool placed in front of the fiber coupling
device. (Refer to Section, "5.4.5 Final Adjustment for
the Ar Laser Light with the Optical Fiber" in Part 1.)
Adjust all the four lasers mounted on the laser unit so
that each laser beam is led into the fiber.
Use the regular procedure for setting up the LU4A four
laser module A without a beamsplitter.
For other three lasers, use the beam shift part and
dichroic mirror adjustment part of each laser.
Note that with respect to the three lasers other than the
reference 488-nm laser, the laser incidence efficiency
in terms of the brightness at the fiber end is about
40%, based on the brightness in front of the coupler.
Manually open the shutter with a tool.
Adjust the additional optical fiber so that the 488-nm
laser beam is led into it, by using the four adjustment
parts of the fiber coupling device only.
Use the procedure for setting up the fiber coupling
device of the C-LU3EX 3 laser unit EX (3).
During the adjustment, do not touch any part other
than the fiber coupling device for additional optical
fiber.
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