Nikon Eclipse TI-TIRF-E Setup Manual page 198

Illuminator unit/motorized illuminator unit/photo activation illuminator unit, beamsplitter 50/50
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Attach the power
meter with a tape.
Figure 5.4-80
Precautions
When some steps of the frequency value cause the same maximum power, the lowest
frequency value is used.
The 1st-order light transmittance of the 405-nm laser, 440-nm laser and 638-nm laser is
lower than that of other lasers; it is as low as approximately 65%.
The transmittance of the 488-nm Ar laser passed through the AOTF is approximately 50%
of the whole Ar laser light.
The transmittance of the 543- or 561-nm laser is approximately 90%.
In case the frequency adjustment has failed, a vertical stripe pattern may periodically
appear in a captured image, at the wavelengths of 543 nm and 561 nm in particular. In that
case, carefully readjust the frequency.
Figure 5.4-81
Chapter 5 Laser Unit
5.4 LU4A Four-laser Module A
In case of the LU4A, remove the fiber coupling and
attach the power meter to the far end of the laser
module body with a tape. It enables you to perform
the AOTF remote controller adjustment while
measuring the laser power.
4.
Subject the sensor of the power meter to the laser
light. Change the [POWER] value by pressing the
arrow key (∧ or ∨) button so that the laser power
value of the power meter becomes maximum.
Calculate the 99% value of the maximum. And then
adjust the [POWER] value so that the laser power
value of the power meter becomes the 99% value of
the maximum.
Precautions
If the [POWER] value is too large, the linearity of
the brightness control will be affected. Be sure to
adjust the [POWER] value so that the laser power
value becomes the 99% value of the maximum.
1-184

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