Nikon Eclipse TI-TIRF-E Setup Manual page 121

Illuminator unit/motorized illuminator unit/photo activation illuminator unit, beamsplitter 50/50
Table of Contents

Advertisement

2
Adjusting the lasers
Adjustment of the argon laser
1.
Mount the dichroic mirror centering tool in place of the dichroic mirror for the argon laser (see
Figures 5.2-5 and 5.2-6).
2.
Open the shutter for the argon laser.
3.
To adjust the laser beam in the horizontal direction, aim the laser beam to the Hole L on the
centering tool by moving the laser body in the range of clearance of the mounting plate. And then
fix the laser body. To adjust the laser beam in the vertical direction, change the vertical position of
the Hole L on the centering tool. The vertical position can be adjusted by rotating the lower part
while holding the upper part of the centering tool. (See Figure 5.2-7.)
4.
After adjustment, tighten the four hexagonal socket-head bolts on the argon laser.
5.
Close the shutter for the argon laser.
Shutter
Adjust the height of
the dichroic mirror
centering tool.
Adjustment of the He-Ne laser
Shutter
Figure 5.2-8
Chapter 5 Laser Unit
5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit
Figure 5.2-6
Rubber cap
Sheet metal cover
Dichroic mirror centering tool
Hole L
Figure 5.2-7
Do not change the height of the dichroic mirror centering
tool after the adjustment of the argon laser. Mount the
centering tool onto one of the positions of dichroic mirrors
for He-Ne lasers as shown in Figure 5.2-9. Adjust the
He-Ne laser beam settings respectively.
1.
Open the shutter for the He-Ne laser.
(There is a red rubber cap at the tip of the optical fiber
for the excitation light. Take the cap and put it
between the shutter and the sheet metal cover to
keep the shutter open. See Figure 5.2-8.)
2.
The mounting hardware at the rear of the He-Ne
laser should be fixed at the center. Aim the laser
beam to the Hole L on the centering tool by adjusting
the adjustment screws of the mounting hardware at
the front of the He-Ne laser. The tolerance of the
positioning is 0.2 mm or better.
3.
Close the shutter for the He-Ne laser.
1-107
1mm
Hole R
1) Hold to prevent
movement
2) Rotate here while
holding the upper
section to adjust the
height

Hide quick links:

Advertisement

Table of Contents
loading

Table of Contents