Nikon Eclipse TI-TIRF-E Setup Manual page 273

TIRF-PAU illuminator
(6)
(7)
Parts used in the TIRF microscopy operation
(1) Optical fiber
(2) Laser position adjustment knob
(for forward and backward adjustment)
(3) Focal point adjustment knob
(4) Condenser lens locking screw
(5) Excitation filter slider
Parts used in the PAU illumination operation
(6) Optical fiber
(7) Laser focus adjustment knob
(8) Laser focus adjustment knob clamp screw
(9) Laser position adjustment knob
(for right and left adjustment)
(10) Laser position adjustment knob
(for forward and backward adjustment)
(11) Excitation filter slider
For switching the optical path between TIRF (or PAU) illumination and epi-fl illumination or adjusting the
focal point of the TIRF illuminator right and left (i.e., TIRF illumination angle), use the TI-RCP remote
control pad (or TI-ERGC ergo controller for Ti-U) or the software.
Chapter 1 System Configurations and Part Names
1.4 Name of Each Part
(10)
(8) (9)
(11) (18)
(19)
(1)
(2)
(3)
(4)
(5)
(13)
Figure 1.4-3
Parts used in the epi-fl microscopy
operation
(12) Aperture diaphragm centering screws
(13) Aperture diaphragm open/close lever
(14) ND filter sliders
(15) Excitation filter slider
(16) Connector for the epi-fl light source
(17) Auxiliary filter holder
Parts used in common to all operation
(18) Field diaphragm centering screws
(19) Field diaphragm open/close lever
(20) Mirror switching lever
(* when the total reflection mirror is
attached)
Push in: mirror in (PAU illumination)
Pull out: mirror out (TIRF illumination)
2-15
(20)
(16)
(14)
(17)
(12) (15)