Nikon Eclipse TI-TIRF-E Setup Manual page 289

2
Attach the dichroic mirrors (photo activation illuminator unit only).
It is necessary to attach a dichroic mirror (optical path switch mirror), which switches between laser light and
episcopic illumination, inside the photo activation illuminator unit. Since the attachment does not ship with a
mirror attached, you must attach one following the procedure below.
You can also change the light intensity ratio of laser light and episcopic illumination by switching the dichroic
mirror and operating the optical path switch lever.
Ordinarily, you should attach a total reflection mirror. When using a total reflection mirror, operating the
optical path switch switches between 100% laser light and 100% episcopic illumination.
WARNING
To prevent hazardous laser emission from the mirror replacement opening, turn off the laser
power before replacing mirrors.
Figure 4.2-2
Holder plate locking screws
Mirror unit
Figure 4.2-3
Chapter 4 Setting Up the Microscope
4.2 Setup Procedure
Cap
Holder plate
Flat spring
Mirror
(Place the
reflection
surface
downward.)
1. Remove the retaining screw for the cap of the
mirror replacement opening using the Allen
wrench, and then remove the cap by turning it
counterclockwise.
2. Remove the mirror unit from inside the
illuminator, and insert the dichroic mirror.
(1) When removing the mirror unit, pull out the
mirror switch lever, and then pull out the
mirror unit while holding the tab of the mirror
unit (magnetic lock).
(2) Remove the two holder plate locking
screws. Next, remove the holder plate and
flat spring to attach the mirror.
• Mirror size: 36 x 25.7 x 1
3. Attach the mirror unit in its original position, and
then close the cap of the mirror replacement
opening.
(1) There is a positioning hole on the mirror
unit. Securely attach the mirror unit to the
illuminator to fit the pin on the main body.
(2) Screw the cap of the mirror replacement
opening until it is closed.
(3) Tighten the optical path switching module
screw using the Allen wrench, and secure
the cover.
2-31