Nikon Eclipse TI-TIRF-E Setup Manual page 241

Chapter 6 Connection between the Laser Unit and the Microscope
Operating the N-STORM optical system
When the optical system for N-STORM microscopy is installed, the ND filter of the laser adapter, the STORM
slider, the lambda plate slider, and the cylindrical lens of the 3D-STORM port must be inserted or removed
according to the microscopy method as shown in the following table.
Microscopy
Laser adapter ND
method
Confocal
microscopy
Epifluorescent
microscopy
TIRF microscopy
2D STORM
3D STORM
(The ND filter is placed within the optical path.)
6.4 Installing the N-STORM Kit
Laser unit
filter
OUT
OUT
OUT
IN
IN
IN
Figure 6.4-2 IN state and OUT state of the laser adapter ND filter
Table 6.4-1
Inverted microscope body
STORM slider Lambda plate slider
Not used
Not used
OUT
IN
IN
(The ND filter is placed out of the optical path.)
1-227
3D-STORM port
cylindrical lens
Not used
Not used
OUT
IN
IN
OUT
Not used
OUT
OUT
OUT
IN