Nikon Eclipse TI-TIRF-E Setup Manual page 196

Illuminator unit/motorized illuminator unit/photo activation illuminator unit, beamsplitter 50/50
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Figure 5.4-74
Vertical-direction setscrew
Figure 5.4-75
Centering tool
Figure 5.4-76
Chapter 5 Laser Unit
5.4 LU4A Four-laser Module A
16. Fully loosen the clamp screw that fixes the setscrew
for the vertical direction adjustment of the L4 dichroic
mirror adjustment part.
17. Aim the laser light at the hole of the centering tool
attached in step 3 in "3. Adjusting the Ar laser
position" by rotating the setscrew for the vertical
direction adjustment of the L4 dichroic mirror
adjustment part.
Centering tool
18. Close the L4 laser shutter.
19. To adjust the 405-nm laser or the 638-nm laser,
select the laser wavelength on the AOTF remote
controller. And then, take the same steps for the
green HeNe laser or the 561-nm solid-state laser.
Rotate the centering tool 90 degrees that was
attached in step 3 in "3. Adjusting the Ar laser
position. The laser light from the 405-nm laser or the
638-nm laser must enter the centering tool at a right
angle.
The laser light must pass through the hole of the
centering tool.
Remove the centering tools.
1-182

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