Nikon Eclipse TI-TIRF-E Setup Manual page 129

Illuminator unit/motorized illuminator unit/photo activation illuminator unit, beamsplitter 50/50
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Chapter 5 Laser Unit
5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit
Figure 5.2-20
3.
If even a small amount of laser light is observed,
adjust the two fiber side adjustment screws on the
coupling to find the point at which the Ar laser light
increases in intensity. (Final fine adjustment will be
performed after adjusting the polarizing orientation.)
4.
Gradually tighten the fiber connector. Stop just before
the point at which light is no longer emitted. Now
adjust the two fiber side adjustment screws to find the
point giving a maximum light intensity. Repeat this
procedure to ensure a maximum intensity, checking
to confirm that the fiber connector is tightened.
5.
If no light is emitted even after repeating these steps,
repeat the procedure from step1 in Section 5.2.4,
"Final Adjustment for the Ar Laser Light with the
Optical Fiber."
6.
Tighten the fiber connector at the point giving a
maximum Ar laser light brightness. Check that there
is no play in the fiber.
7.
Close the Ar laser shutter.
1-115

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