Nikon Eclipse TI-TIRF-E Setup Manual page 202

Illuminator unit/motorized illuminator unit/photo activation illuminator unit, beamsplitter 50/50
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3
Aligning each laser beam to the Ar laser beam
In this section, the green HeNe laser and the 561-nm solid-state laser are described as examples.
1.
Put a target such as a piece of paper at 50 cm or farther away from the outlet of the laser of the
optical fiber coupling device.
Figure 5.4-88
Figure 5.4-89
Figure 5.4-90
6.
Repeat Step 4 and Step 5. Laser spots on the target and the mirror part must be aligned.
7.
Close the L3 laser shutter and L4 laser shutter.
8.
Repeat Step 1 to Step 7 for the 405-nm laser and the 605-nm laser.
Chapter 5 Laser Unit
5.4 LU4A Four-laser Module A
2.
3.
4.
Align two spots.
5.
Align two spots.
1-188
Emit the Ar laser and the green HeNe laser or
561-nm solid-state laser together by using the AOTF
remote controller.
• To align the green HeNe laser to the Ar laser:
Press the [3] and [5] keys in this order while the
screen of the AOTF driver remote controller
displays "internal mode." The 488-nm Ar laser and
the G-HeNe laser are emitted together.
• To align the 561-nm solid-state laser to the Ar
laser:
Press the [3] and [6] keys in this order while the
screen of the AOTF driver remote controller
displays "internal mode." The 488-nm Ar laser and
the 561-nm solid-state laser are emitted together.
Manually open the L3 laser shutter and L4 laser
shutter.
Observe the two spots on the target. One spot is the
light of the Ar laser. The other spot is the light of the
green HeNe laser or the 561-nm solid-state laser.
Adjust the L4 dichroic mirror adjustment part so that
the two spots converge. (Do not touch any part of the
Ar laser optical path.) For details about the
adjustment, refer to Steps 15 to Step 17 <for LU4> in
"4. Adjusting other lasers" in Section 5.4.3, "Laser
Rough Adjustment 1.")
Observe the two spots on the mirror part. Two spots
here must converge to the same point, too. Adjust
the L4 beam shift part of the laser to be aligned. (Do
not touch any part of the Ar laser optical path.) For
details about the adjustment, refer to Steps 7 to Step
10 <for LU4> in "4. Adjusting other lasers" in Section
5.4.3, "Laser Rough Adjustment 1.")

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