Nikon Eclipse TI-TIRF-E Setup Manual page 16

Illuminator unit/motorized illuminator unit/photo activation illuminator unit, beamsplitter 50/50
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4.
Setting Up the Microscope ................................................................................................................ 1-71
4.1 List of Parts .................................................................................................................................. 1-71
4.2 Setup Procedure .......................................................................................................................... 1-74
4.2.1
Assembling Each Part and Attaching Them to the Microscope....................................... 1-74
4.2.2
Attaching the N-STORM Kit ............................................................................................. 1-85
4.2.3
Attaching the Laser Safety Kit.......................................................................................... 1-87
4.2.4
Attaching the Stage Up Kit ............................................................................................... 1-89
4.2.5
Attaching the Back Port Unit ............................................................................................ 1-92
5.
Laser Unit ............................................................................................................................................ 1-93
5.1 Overview of the Laser Units ......................................................................................................... 1-94
5.1.1
AOM Controller (Optional) ............................................................................................. 1-100
5.1.2
LU-LR Four-laser PS Rack ............................................................................................ 1-101
5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit .................................................................. 1-103
5.2.1
Preparation..................................................................................................................... 1-103
5.2.2
Laser Installation............................................................................................................ 1-104
5.2.3
Laser Rough Adjustment................................................................................................ 1-106
5.2.4
Final Adjustment for the Ar Laser Light with the Optical Fiber....................................... 1-113
5.2.5
with the Optical Fiber ..................................................................................................... 1-119
5.2.6
Attaching the Sheet Metal Covers ................................................................................. 1-121
5.3 C-LU3EX Three-laser Unit EX ................................................................................................... 1-122
5.3.1
Preparation..................................................................................................................... 1-123
5.3.2
Laser Installation............................................................................................................ 1-125
5.3.3
Laser Rough Adjustment 1............................................................................................. 1-128
5.3.4
Laser Rough Adjustment 2............................................................................................. 1-139
5.3.5
Final Adjustment for the Ar Laser Light with the Optical Fiber....................................... 1-146
5.3.6
with the Optical Fiber ..................................................................................................... 1-152
5.3.7
Adjusting the AOM Controller (When the AOM Unit Used Only) ................................... 1-154
5.3.8
Attaching the Sheet Metal Cover ................................................................................... 1-155
5.4 LU4A Four-laser Module A ......................................................................................................... 1-156
5.4.1
Preparation..................................................................................................................... 1-157
5.4.2
Laser Installation............................................................................................................ 1-161
5.4.3
Laser Rough Adjustment 1............................................................................................. 1-171
5.4.4
Laser Rough Adjustment 2............................................................................................. 1-183
5.4.5
Final Adjustment for the Ar Laser Light with the Optical Fiber....................................... 1-189
5.4.6
Final Adjustment for Other Laser Light with the Optical Fiber ....................................... 1-194
5.4.7
Removing the AOTF Driver Remote Controller ............................................................. 1-196
5.4.8
Attaching the Sheet Metal Cover ................................................................................... 1-197
5.5 Setting Up the LU-LR Four-laser PS Rack ................................................................................ 1-198
5.5.1
System Description ........................................................................................................ 1-198
5.5.2
Mounting Laser Power Supply....................................................................................... 1-201
5.5.3
Details on Mounting Laser Supply ................................................................................. 1-207
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