Nikon Eclipse TI-TIRF-E Setup Manual page 67

Optical path selector switch
Figure 1.4-31
N-STORM
Lambda plate
slider
STORM slider
DSC support column
3D-STORM port
Combination of A1 and 3D-STORM port
Figure 1.4-32
Chapter 1 System Configurations and Part Names
1.4 Name of Each Part
Optical path selector switch
Ti-E fixing
plate
Cylindrical lens
IN/OUT handle
OUT
IN
3D-STORM port
The laser shutter does not open when the
nosepiece tube route of the optical path ("EYE") is
selected.
Ti-E
EYE Observation port 100%
L100 Left port 100%
L80 Left port 80% and observation port 20%
R100 Right port 100%
Ti-E/B
EYE Observation port 100%
L100 Left port 100%
L80 Left port 80% and observation port 20%
B100 Bottom port 100%
The 3D-STORM port connecting the Ti-E
microscope and EM-CCD camera when performing
the N-STORM microscopy is equipped with the
cylindrical lens that is placed in or out of the optical
imaging system to the camera by sliding the
handle. In normal microscopy and 2D-STORM
microscopy, remove the cylindrical lens out of the
optical path, and in capturing the 3D-STORM
image, insert it in the optical path.
If the cylindrical lens is installed during normal
microscopy and 2D-STORM microscopy, images
are distorted. In 3D-STORM microscopy, the shape
of each bright spot changes to oval.
(When using this port together with the confocal
microscope A1, install the 3D-STORM port in the
right port. When using this port independently,
install it in the left port.)
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