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ECLIPSE Ni-E
Nikon ECLIPSE Ni-E Manuals
Manuals and User Guides for Nikon ECLIPSE Ni-E. We have
4
Nikon ECLIPSE Ni-E manuals available for free PDF download: Instruction Manual
Nikon ECLIPSE Ni-E Instruction Manual (186 pages)
Upright Motorized Microscope
Brand:
Nikon
| Category:
Microscope
| Size: 54.45 MB
Table of Contents
Introduction
3
Contents of the Manual
4
Symbols Used in this Manual
4
Differential Interference Contrast
5
Summary of Contents
5
Table of Contents
6
Safety Precautions
8
WARNING and CAUTION Symbols
8
Meaning of Symbols Used on the Product
8
Warning
8
Caution
8
Notes on Handling the Product
13
Components
15
System Configuration and Controls for Bright/Dark-Field Microscopy
16
Components
16
Controls
17
Buttons
18
Connectors
19
System Configuration and Controls for Epi-Fluorescence Microscopy
20
System Configuration and Controls for Differential Interference Contrast Microscopy
22
System Configuration and Controls for Phase Contrast Microscopy
23
Microscopy Operations
25
Before Microscopy
26
Operation Flowchart
28
Bright/Dark-Field Microscopy Procedure
32
Lower the Condenser Slightly from the Uppermost Position
34
Fully Open the Field Diaphragm and Aperture Diaphragm
35
Focus on the Sample
38
Adjust the Interpupillary Distance
39
Focus and Center the Condenser
39
Oil Immersion
41
Adjust the Aperture Diaphragm
42
Adjust the Field Diaphragm
43
Epi-Fluorescence Microscopy Procedure
47
Differential Interference Contrast Microscopy Procedure
63
Phase Contrast Microscopy Procedure
81
Individual Operations
97
Display Panel Details
98
Using Operation Buttons on Ni-E
101
Front Operation Button
101
Right Operation Button
102
Left Operation Button
103
Adjusting the Brightness of a Diascopic Image
104
Adjustment by Lamp Voltage
104
Adjustment with ND Filters
105
Focusing on the Sample (Vertical Stage Movement)
106
Proper Focusing Procedure
106
Focus Knobs on the Main Body
107
Resetting the Z-Axis Coordinate
108
Refocusing
109
Bringing the Target into the Optical Path (Horizontal Stage Movement, Rotation)
110
Operating the Rotatable Ceramic Coated Stage
110
Operating the Motorized XY Stage
112
Adjusting the Diopter
113
Focusing and Centering the Condenser
114
Adjusting the Aperture Diaphragm
115
Using the Condenser
117
Compatibility of Condensers with the Magnification of Objectives
117
Using the NI-CUD-E Motorized Universal Condenser (Dry)
118
Using the NI-CUD Universal Condenser (Dry)
119
Using the D-CUO DIC Condenser (Oil)
121
Adjusting the Field Diaphragm
122
Switching the Objective
123
Motorized Nosepiece Operation
123
Intelligent/Manual Nosepiece Operation
125
Switching the Optical Path of the Tube
126
Optical Path Switching of the Motorized Quadrocular Tilting Tube
126
Switching the Optical Path in a Manually-Operated Tube
127
Adjusting the Binocular Section
128
Adjusting with the Binocular Section of the Tube
128
Using the Eyelevel Riser
128
Oil/Water Immersion
129
Dark-Field Microscopy
131
Epi-Fluorescence Microscopy
132
Epi-Fluorescence Cube Turret Operation (Switching Excitation Methods)
132
Selecting Filters
134
Protecting the Sample and Preventing It from Decoloration (Using the Shutter)
136
Adjusting the Brightness of the Fluorescent Image (Using ND Filters and the Aperture Diaphragm)
138
Restricting the Illumination to the Area of the Specimen Being Viewed (Centering and Adjusting the Field Diaphragm)
140
Changing the Waveform Characteristics of the Excitation Light (D-FB Excitation Balancer)
141
Switching the Excitation Filter (Motorized Excitation Filter Wheel)
142
Switching the Barrier Filter (Motorized Barrier Filter Wheel)
143
Other Notes on Epi-Fluorescence Microscopy
143
Differential Interference Contrast Microscopy
144
Tips for Differential Interference Contrast Microscopy
144
Using Optical Elements
145
Phase Contrast Microscopy
146
Tips for Phase Contrast Microscopy
146
Using Optical Elements
147
Useful Functions
148
Mode
148
Interlocking Function
152
Using the Ergo Controller
155
Using the Joystick Controller
158
Capturing Images
159
Operation on DS-L3
164
Setting up the Microscope
164
Controlling the Microscope
180
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Nikon ECLIPSE Ni-E Instruction Manual (120 pages)
Upright motorized microscope (Focusing Nosepiece System)
Brand:
Nikon
| Category:
Microscope
| Size: 30.73 MB
Table of Contents
Table of Contents
6
Introduction
3
Contents of the Manual
4
Symbols Used in this Manual
4
Summary of Contents
5
Table of Contents
6
Safety Precautions
8
Caution
8
Meaning of Symbols Used on the Product
8
Warning
8
WARNING and CAUTION Symbols
8
Notes on Handling the Product
13
Components
15
Ni-E Focusing Nosepiece System Basic Configuration
16
Components
16
Controls
17
Operation Buttons
18
Connectors
19
Microscopy Operations
21
Before Microscopy
22
Operation Flowchart
24
Table of Contents
25
IR-DIC (Infrared Ray Differential Interference Contrast) Microscopy Procedure
26
Turn on the Power
26
Turn on the Dia-Illumination Lamp
27
Lower the Condenser Slightly from the Uppermost Position
28
Fully Open the Field Diaphragm and Aperture Diaphragm
29
Bring the 4X or 10X Objective into the Optical Path
31
Focus and Orientation
31
Place a Sample on the Stage, and Move the Stage to Bring the Target into View
31
Focus on the Sample
32
Adjust the Interpupillary Distance
33
Perform Diopter Adjustment
33
Focus and Center the Condenser
34
Adjust the Aperture Diaphragm
35
Adjust the Field Diaphragm
36
Adjust the Orientation (Vibration Direction) of the Polarizer and Analyzer
38
Observe a Sample Using a Microscope
40
Diagonal Illumination
42
Epi-Fluorescence Microscopy Procedure
43
Close the Shutter and Block the Illumination Path
44
Bring the Filter Cube into the Optical Path
45
Turn on the Mercury Lamp
46
Microscopy with Different Magnification
46
Open the Shutter
47
Turn off the Mercury Lamp
50
Individual Operations
55
Display Panel Details
56
Using Operation Buttons on Ni-E
59
Front Operation Button
59
Right Operation Button
60
Left Operation Button
61
Adjusting the Brightness of a Diascopic Image
62
Adjustment by Lamp Voltage
62
Adjustment with ND Filters
63
Focusing on the Sample (Vertical Objective Movement)
64
Proper Focusing Procedure
64
Focus Knobs on the Main Body
65
Resetting the Z-Axis Coordinate
66
Refocusing
67
Bringing the Target into the Optical Path (Horizontal Stage Movement)
68
Standard Stage Operation
68
Operating the Motorized XY Stage
69
Adjusting the Diopter
70
Focusing and Centering the Condenser
71
Adjusting the Aperture Diaphragm
72
Using the Condenser
73
Adjusting the Field Diaphragm
75
Operating the Nosepiece (Objective Operation)
76
Objective Vertical Movement Lever (Objective Switching, Dipping)
76
Parfocal Correction of Objective
77
Centering the Objective
78
Inserting/Removing the DIC Slider
78
Single Nosepiece
78
Using the Polarizer Turret
79
Switching the Optical Path of the Tube
79
Adjusting the Binocular Section
80
Water Immersion
80
Differential Interference Contrast Microscopy
81
Tips for Differential Interference Contrast Microscopy
81
Using Optical Elements
82
Epi-Fluorescence Microscopy
83
Epi-Fluorescence Cube Turret Operation (Switching Excitation Methods)
83
Selecting Filters
85
Protecting the Sample and Preventing It from Decoloration (Using the Shutter)
87
Adjusting the Brightness of the Fluorescent Image (Using ND Filters and the Aperture Diaphragm)
89
Restricting the Illumination to the Area of the Sample Being Viewed (Centering and Adjusting the Field Diaphragm)
90
Changing the Waveform Characteristics of the Excitation Light (D-FB Excitation Balancer)
91
Other Notes on Epi-Fluorescence Microscopy
92
Useful Functions
93
Mode Function
93
Interlocking Function
96
Capturing Images
97
Operation on DS-L3
101
Setting up the Microscope
101
Microscope Control
114
Nikon ECLIPSE Ni-E Instruction Manual (72 pages)
Upright motorized microscope
Brand:
Nikon
| Category:
Microscope
| Size: 15.3 MB
Table of Contents
Introduction
3
Contents of the Manual
4
Symbols Used in this Manual
4
Specifications
5
Summary of Contents
5
Table of Contents
6
Assembly
9
ECLIPSE Ni-E System Configuration
10
Components List
12
Assembly Method
14
Introduction : Motorized Device Combination
14
Check Input Voltage
15
Unfasten the Elevating Section
15
Escape the Elevating Section
16
Attach the Motorized ND Filter Wheel (Optional)
16
Attach the DIA Motorized Shutter (Optional)
16
Attach the Lamp
17
Attach the Dia-Illumination Lamphouse
17
Attach the Contact Arm/Standard Arm
18
Attach the Connector Box (Optional)
18
Attach the Epi-Fluorescence Cube Turret and Epi-Fluorescence Attachment (Required for Epi-Fluorescence Microscopy)
19
Attaching the Filter Cube
20
Replacing Excitation and Barrier Filters
21
Attaching the Light Shielding Plate
21
Attach the Motorized Barrier Filter Wheel (Optional)
22
Attaching the Barrier Filter
23
Attach the Quadrocular Tilting Tube and DSC Zooming Port for Quadrocular Tube
24
Attach the Motorized Excitation Filter Wheel (Optional)
25
Attaching the Excitation Filter
26
Attach the EPI Motorized Shutter (Optional)
26
Attach Eyepieces
26
Attach the Substage
27
Attach the Stage
28
[17.1] Rotatable Ceramic Coated Stage
28
[17.2] Motorized XY Stage
28
Attach the Condenser
31
[18.1] Motorized Universal Condenser
31
[18.2] Manual Condenser (with the Condenser Holder)
33
Attaching the Optical Module to the Universal Condenser
33
Attaching the DIC Module to DIC Condenser
37
Attach the Nosepiece
39
Attach the Objective
39
Attach the DIC Rotation Polarizer Unit (Required for Differential Interference Contrast Microscopy)
39
Attach the Eyelevel Riser (Optional)
40
Attach a Camera and Connect the DS Camera Control Unit (Optional)
40
Quadrocular Tube
40
Binocular Tube
41
[23.3] Installing a Camera Head to the Quadrocular Tilting Tube
41
[23.4] Installing a Camera Head to the Trinocular Tube
41
Replace the ND Filter
42
Connect the Motorized Unit Cable
42
Connect the Power Cord
43
Troubleshooting
45
Optical System and Operation
46
General
46
Epi-Fluorescence Microscopy
50
Differential Interference Contrast Microscopy
51
Phase Contrast Microscopy
52
Electrical System
53
General
53
Epi-Fluorescence Microscopy
59
Maintenance and Storage
61
Replacing the Lamp
62
Cleaning
63
Cleaning Lenses
63
Cleaning Parts Other than the Lens
63
Cleaning Immersion Oil
64
Decontaminating the Product
64
Restoring Microscope Data to Factory Default
64
Transportation (Using the Fastening Position Mode Switch)
65
Storage
65
Periodic Inspections (Charged)
66
Specifications
67
Microscopy (Principles)
68
Performance Properties
68
Physical Properties
71
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Nikon ECLIPSE Ni-E Instruction Manual (57 pages)
Upright Motorized Microscope, Focusing Nosepiece System
Brand:
Nikon
| Category:
Microscope
| Size: 12.25 MB
Table of Contents
Introduction
3
Contents of the Manual
4
Symbols Used in this Manual
4
Troubleshooting
5
Summary of Contents
5
Table of Contents
6
Assembly
9
ECLIPSE Ni-E (Focusing Nosepiece System) System Configuration
10
Components List
12
Assembly Method
14
Introduction: Motorized Device Combination
14
Check Input Voltage
15
Unfasten the Elevating Section
15
Attach the Lamp
16
Attach the Lamphouse for Dia-Illumination
16
Attach the Focusing Nosepiece Sub Arm
17
Attach the Focusing Nosepiece Substage
17
Attach the Polarizer Turret (Required for Differential Interference Contrast Microscopy)
18
Attaching Optical Module
18
Attach the Nosepiece
19
Attach the Condenser
20
Attaching the Optical Module to the Condenser Turret
20
Attach the Stage
22
[10.1] Standard Stage
22
[10.2] Motorized XY Stage
23
Attach the Arm Spacer
27
Attach the Contact Arm/Standard Arm
28
Attach the Connector Box (Optional)
28
Attach the Epi-Fluorescence Cube Turret and Epi-Fluorescence Attachment (Required for Epi-Fluorescence Microscopy)
29
Attaching the Filter Cube/Analyzer Cube
30
Replacing Excitation and Barrier Filters
31
Attaching a Light Shielding Plate
31
Attach the Quadrocular Tilting Tube and DSC Zooming Port for Quadrocular Tube
32
Attach Eyepieces
33
Attach the Objective
33
[17.1] Slider Nosepiece
33
[17.2] Single Nosepiece
33
Attach the Camera and DS Camera Control Unit (Optional)
34
[18.1] Attaching to DSC Zooming Port for Quadrocular Tube
34
[18.2] Attaching to the Quadrocular Tilting Tube
34
Replace the ND Filter (Optional)
35
Connect the Motorized Device Cable
35
Connect the Power Cord
36
Troubleshooting
37
Optical System and Operation
38
General
38
Differential Interference Contrast Microscopy
41
Epi-Fluorescence Microscopy
42
Electrical System
43
General
43
Epi-Fluorescence Microscopy
46
Maintenance and Storage
47
Replacing the Lamp
48
Cleaning
49
Cleaning Lenses
49
Cleaning Parts Other than the Lens
49
Cleaning Immersion Oil
50
Decontaminating the Product
50
Restoring Microscope Data to Factory Default
50
Transportation (Using the Fastening Position Mode Switch)
51
Storage
51
Periodic Inspections (Charged)
51
Specifications
53
Microscopy (Principles)
54
Performance Properties
54
Physical Properties
56
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