Nikon Eclipse TI-TIRF-E Setup Manual page 158

Illuminator unit/motorized illuminator unit/photo activation illuminator unit, beamsplitter 50/50
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6
Fine-adjusting the angle of the other two dichroic mirror adjustment units and adjusting the
beam shift unit
Figure 5.3-52
Chapter 5 Laser Unit
5.3 C-LU3EX Three-laser Unit EX
1.
Adjust the angle of the dichroic mirror adjustment
unit and align the He-Ne laser beam position with the
target positioned at a distance of 50 cm.
Since the cross-section of the 405 cylindrical laser
beam is upright elliptical, estimate a center point and
use this for alignment.
1-144

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