Nikon Eclipse TI-TIRF-E Setup Manual page 288

1
TIRF-PAU illuminator installation
1. Remove the clamping screws 1 and 2 from the part used in PAU illumination operation.
2. Press the part used in PAU illumination operation into the microscope to secure it.
3. Attach the two support legs (for two areas) on the part used in PAU illumination operation to
adjust the height.
4. Attach both parts used in TIRF-microscopy operation and for PAU illumination operation to
secure them. (Remove the clamping screw 1 in advance, or you may fail to attach the parts.)
5. Attach both the optical fibers for the parts used in TIRF-microscopy operation and PAU
illumination operation. Adjust the height of each part to approx. 110 mm as shown in the figure
to secure it.
6. Perform optical adjustments for the parts used in TIRF-microscopy operation and PAU
illumination operation.
This is the end of the TIRF-PAU illuminator installation.
Optical fiber for TIRF
TIRF illuminator unit
Fixing screw 1
PAU illuminator unit
Support legs
Fiber for PAU
Fixing screw 2
Chapter 4 Setting Up the Microscope
4.2 Setup Procedure
Approx.
110 mm
Figure 4.2-1
2-30