Nikon Eclipse TI-TIRF-E Setup Manual page 152

Illuminator unit/motorized illuminator unit/photo activation illuminator unit, beamsplitter 50/50
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PWR adjustment - for the new AOM
New AOM (Checkpoint: the nameplate on the front indicates NIKON only. and the serial number on
the nameplate is 59001 or higher)
1.
Connect each part, then set the laser power to 100% on NIS-Elements.
2.
Measure the 1st-order light intensity using the power meter to determine the maximum
brightness.
Insert a fine screwdriver (like the screwdriver provided with the PCI-6711) into the PWR volume
and turn it clockwise very slowly.
The maximum brightness will be achieved almost immediately for Ar lasers. G-HeNe lasers will
require a small amount of rotation, and R-HeNe lasers somewhat more.
3.
Turn the PWR volume counter-clockwise from the position of the maximum brightness.
For Ar and G-HeNe lasers, reduce to approximately 98% of the maximum brightness. For
R-HeNe lasers, reduce to approximately 80% of the maximum brightness.
PWR adjustment should be finalized where the volume is turned in the clockwise direction. To
make fine adjustments, lightly rotate the volume counter-clockwise, then slowly turn it back in the
clockwise direction.
(This adjustment is required to prevent too much power from being applied to the AOM
elements.)
Stop at the limit position if the brightness does not fall to 98% when the volume is turned fully
counter-clockwise for the Ar laser.
4.
Perform BIAS volume of AOM driver.
• Check that BIAS adjustment is turned anti-clockwise.
• Set the laser power to 0% on NIS-Elements.
Turn BIAS adjustment clockwise to the minimum brightness.
5.
For multiple AOMs, perform PWR adjustments for other Ar lasers.
This completes the new AOM adjustment.
Next, proceed to Section 5.3.4, "Laser Rough Adjustment 2" using the 1st-order light from each
laser.
Chapter 5 Laser Unit
5.3 C-LU3EX Three-laser Unit EX
1-138

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