Nikon Eclipse TI-TIRF-E Setup Manual page 163

Illuminator unit/motorized illuminator unit/photo activation illuminator unit, beamsplitter 50/50
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4
Adjusting the fiber polarizing orientation
Figure 5.3-63
Figure 5.3-64
Coupling clamping screw
Figure 5.3-65
Figure 5.3-66
Chapter 5 Laser Unit
5.3 C-LU3EX Three-laser Unit EX
1.
Align the knob on the polarizer-equipped tool with
the index. Mount the polarizer-equipped tool on the
polarizing orientation adjustment tool with the knob
at the top.
2.
Clamp the emission end of the optical fiber to the
polarizing orientation adjustment tool. Place a
light-scattering target (such as paper) before it.
Position the fiber so that it is not subjected to
excessive force, and so that both ends are laid out
straight.
3.
Open the Ar laser shutter. Insert a tool into the
coupling and rotate while monitoring the laser light
image on the target to find the point giving the
minimum brightness.
(If the three screws on the coupling are too tight,
loosen them slightly before rotating.)
4.
Place the power meter in front of the polarizer to
measure the laser power.
5.
Rotate the polarizer-equipped tool by 90 degrees so
that the knob faces to the side, place the power
meter in front of the polarizer, and measure laser
power.
6.
Check that the extinction ratio (ratio of bright to dark
laser power) is at least 20:1.
If not, repeat the adjustment procedure from step 1.
7.
Tighten the three clamping screws on the coupling.
1-149

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