Nikon Eclipse TI-TIRF-E Setup Manual page 151

Illuminator unit/motorized illuminator unit/photo activation illuminator unit, beamsplitter 50/50
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8
Adjusting the AOM driver
PWR adjustment - for the conventional AOM
Figure 5.3-37
3.
Turn the PWR volume counter-clockwise from the position of the maximum brightness.
For Ar and G-HeNe lasers, reduce to approximately 98% of the maximum brightness. For
R-HeNe lasers, reduce to approximately 80% of the maximum brightness.
PWR adjustment should be finalized where the volume is turned in the clockwise direction. To
make fine adjustments, briefly rotate the volume counter-clockwise, then slowly turn it back in the
clockwise direction.
(This adjustment is required to prevent too much power from being applied to the AOM
elements.)
Stop at the limit position if the brightness does not fall to 98% when the volume is turned fully
counter-clockwise for the Ar laser.
4.
Perform BIAS adjustment of AOM driver.
• Record the brightness when the laser power is set to 100%.
• Set the laser power to 50% on NIS-Elements., then turn BIAS volume counter-clockwise to
reduce the brightness to half.
5.
For multiple AOMs, perform PWR adjustments for other Ar lasers.
This completes the conventional AOM adjustment.
Next, proceed to Section 5.3.4, "Laser Rough Adjustment 2" using the 1st-order light from each
laser.
Chapter 5 Laser Unit
5.3 C-LU3EX Three-laser Unit EX
1.
Connect each part, then set the laser power to 100%
on NIS-Elements.
2.
Measure the 1st-order light intensity using the power
meter to determine the maximum brightness.
Insert a fine screwdriver (like the screwdriver
provided with the PCI-6711) into the PWR volume
and turn it clockwise very slowly.
The maximum brightness will be achieved almost
immediately for Ar lasers. G-HeNe lasers will require
a small amount of rotation, and R-HeNe lasers
somewhat more.
1-137

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