Nikon Eclipse TI-TIRF-E Setup Manual page 125

Illuminator unit/motorized illuminator unit/photo activation illuminator unit, beamsplitter 50/50
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8
Centering the He-Ne1 laser beam in the middle
If the He-Ne1 laser is located in the middle of the enclosure, adjust its dichroic mirror so that the
He-Ne1 laser beam aligns with the pinhole of the target tool set in the previous step. The accuracy
should be within 0.8 mm.
9
Checking the screws
Make sure that all the dichroic mirror adjustment screws are firmly tightened.
10
Aligning polarization of lasers
This section describes how to align the direction of polarization of the He-Ne laser with reference to that
of the argon laser. (See Figure 5.2-13.)
1.
Place a polarizer-equipped tool at the end of the argon-laser dichroic mirror.
At this time, place the polarizer-equipped tool with its flat surface at the back, as shown in Figure
5.2-13.
2.
Place a light-scattering target (such as paper) at the end of the coupler and then move the knob
on the polarizer-equipped tool to determine the position at which the laser beam is the darkest. (At
this time, remove the two ND filters from the argon laser. This will make it easier to locate the
darkest position.)
At this time, the direction of polarization of the argon laser is as shown in Figure 5.2-13.
3.
Place a light-scattering target (such as paper) at the end of the fiber mount and then rotate the
He-Ne laser to determine the position at which the laser beam is the darkest. (At this time, remove
the two ND filters from the He-Ne laser. This will make it easier to locate the darkest position.) At
this time, the polarizer must be oriented in the same direction as that of the argon laser.
At this time, the direction of polarization of the He-Ne laser is as shown in Figure 5.2-13, where the
polarization directions of argon laser and He-Ne laser are aligned. Adjust carefully within the
range of approximately ±2 degrees. (A discussion of the direction of laser polarization is included
in the laser instruction manual. Refer to the manual for rough configuration of the lasers. This will
make it easier to determine the direction of polarization.)
4.
For two He-Ne lasers system, adjust the polarization direction of the second laser in the same
manner.
5.
Rotating the He-Ne laser may displace it from the pinhole of the target tool adjusted as directed in
"6 Centering the He-Ne laser" in this section. If this occurs, adjust the setscrews L1 and R1
provided at the He-Ne laser radiation side as shown in Figure 5.2-13 to align the laser with the
target.
Chapter 5 Laser Unit
5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit
1-111

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