Nikon Eclipse TI-TIRF-E Setup Manual page 124

Illuminator unit/motorized illuminator unit/photo activation illuminator unit, beamsplitter 50/50
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6
Centering the He-Ne laser
As shown in Figure 5.2-12, place the target tool at a distance of about 50 cm from the coupler. To
perform adjustment of the target tool, move its pinhole so that the beam of the He-Ne laser (He-Ne2
with the three-laser unit, He-Ne1 with the two-laser unit) farthest from the argon laser is aligned with the
pinhole of the target tool. The pinhole used for centering should be slightly narrower than the laser
beam. The centering accuracy should be within 0.1 mm.
The target tool position can be adjusted with the two setscrews. The setup condition of the target tool
varies by installation location. When selecting a location, make sure that the laser beam can reach the
target tool.
3) Adjust the dichroic
mirror for the
He-Ne1 laser and
align with the
pinhole
2) Adjust the
dichroic mirror for
the argon laser
and align with the
pinhole
50 cm
7
Centering the argon laser beam
Align the argon laser beam with the pinhole of the target tool that has been adjusted with the He-Ne2
laser.
Adjust the dichroic mirror for the argon laser so that the argon laser beam aligns with the pinhole of the
target tool that has been adjusted with the He-Ne2 laser. The accuracy should be within 0.4 mm.
WARNING
Do not look directly at the incident laser light during adjustment.
Chapter 5 Laser Unit
5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit
1) Target tool
Align the pinhole
position with the
He-Ne2 laser beam
Target tool
Figure 5.2-12
1-110
He-Ne2
Ar
He-Ne1
Centering screw
Pinhole

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