Appendix C Supplemental Procedures; Set Up And Test The Ion Chip ™ Minifuge; Install The Ion S5 ™ /Ion Proton ™ Rotor And Buckets - Thermo Scientific Ion GeneStudio S5 User Manual

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C
Set up and test the Ion Chip
Install the Ion
S5
/Ion Proton
Rotor and Buckets
48
Supplemental procedures
Minifuge
Note: The Ion Chip
Minifuge is only required when using the Ion OneTouch
System for template preparation. Ion Chef
Chip
Minifuge.
The Ion Chip
Minifuge (Cat. No. 4479672 or 4479673) is used to load sequencing
chips manually for use on Ion PGM
platforms. To accommodate the larger chip size, Ion Proton
sequencer users must:
• install the Ion S5
/Ion Proton
• test the minifuge to confirm that no liquid is lost during centrifugation.
before using the minifuge to load chips for the first time.
Note: The following protocols may also be used to convert the Ion Chip
back for use with Ion PGM
1. Grasp the existing rotor and pull straight up to remove the rotor from the motor
shaft.
Motor shaft
1
Instrument users do not require an Ion
, Ion Proton
Rotor and Buckets (Cat. No. 4482578).
sequencing chips.
Ion GeneStudio S5 Instrument User Guide
, and Ion GeneStudio S5 sequencing
and Ion GeneStudio S5
2
Minifuge

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