Perform An Instrument Reset Run With An Initialized, Unused Sequencing; Reagents Cartridge - Thermo Scientific Ion GeneStudio S5 User Manual

3
Chapter 3 Instrument operation
Maintain the sequencer
Perform an
instrument reset
run with an
initialized, unused
Sequencing
Reagents
cartridge
18
Cleaning is normally performed at completion of a sequencing run automatically. If
an Ion GeneStudio S5 is initialized and
• a sequencing run is not started within 24 hours after initialization, or
• a sequencing run is not completed due to a power failure or an abort, and <200
flows occurred before the stoppage
an instrument reset run is required to ensure proper cleaning before reinitialization.
Do NOT perform a manual cleaning with an unused, initialized Ion S5

Reagents cartridge.

Note:
·
If a power failure or abort occurs during the second of two runs started after a
single initialization, a manual cleaning (page 16) is sufficient.
·
If the number of flows that occurred before a power failure or abort is unknown,
perform an instrument reset run.
To perform an instrument reset run, use the following procedure before
reinitialization:
1. In the instrument touchscreen main menu, press Run.
The instrument door unlocks and the chip clamp disengages.
2. Ensure that a used sequencing chip is in the chip clamp, then push the chip
clamp in all the way to engage.
3. Close the instrument door, then press Next.
4. When prompted, select Planned Run (none). Ensure that the Enable post-run
clean checkbox is selected, then press Review.
5. In the Select Run screen, press Edit, then in the Detail screen set the number of
flows to 200. Ensure that the Post-Run/Clean checkbox is selected, then press
Close.
6. Press Start run, then press Accept to confirm that Post-Run Clean is enabled, and
to start the run.
When the instrument reset run completes, the instrument automatically performs the
cleaning procedure. After cleaning, the touchscreen returns to the main menu.
™
Ion GeneStudio S5 Instrument User Guide
Sequencing