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Preparing and Running the Micro Fluidic Cards
(PCR)
Summary
Where You Are in
the Procedure
You
Are Here
Applied Biosystems Micro Fluidic Card Getting Started Guide
Amplification of cDNA is the second step in the two-step RT-PCR
experiment. In this step, the sample-specific PCR mix is loaded into a
Micro Fluidic Card. The Micro Fluidic Card is run on the 7900HT
system, where AmpliTaq Gold
cDNA synthesized from the original total RNA sample.
Performing Reverse Transcription
(cDNA generation)
Preparing and Running the Micro
Fluidic Cards (PCR)
Performing Real-Time Data Analysis
(7900HT system and SDS software)
Interpreting Results
June 23, 2003 9:39 am, MFC_QSG_text.fm
Preparing and Running the Micro Fluidic Cards (PCR)
®
DNA polymerase amplifies the
DRAFT
Loading, Centrifuging,
Sealing, Trimming, and
Running the Micro Fluidic
Cards
1. Prepare the cDNA samples
for the real-time PCR run
(sample-specific PCR mix).
2. Pipette the sample-
specific PCR mix into a
Micro Fluidic Card.
3. Centrifuge the Micro
Fluidic Card.
4. Seal the Micro Fluidic Card
and trim-off fill reservoirs.
5. Create a Micro Fluidic
Card document.
– If you are using SDS
2.1 Software to analyze
results, create a relative
quantification
document.
– If you are using custom
software to analyze
results, create an
absolute quantification
document.
6. Perform the run.
15
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