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Introduction
About the Getting
Started Guide
About the Micro
Fluidic Card
4
June 23, 2003 9:39 am, MFC_QSG_text.fm
This guide accompanies the Micro Fluidic Card. It provides
instructions for the preparation, execution, and analysis of Micro
Fluidic Card experiments.
If you are familiar with the theory behind the Applied Biosystems
7900HT Micro Fluidic Card (Micro Fluidic Card) chemistry or the
®
ABI P
7900HT Sequence Detection System (7900HT system)
RISM
data collection, the information in this document serves as a quick
reference guide for conducting experiments using the Micro Fluidic
Card.
If this is the first time you are using the Micro Fluidic Card, this
guide refers you to the appropriate sections of the ABI P
7900HT Sequence Detection Systems and SDS Enterprise Database
User Guide (PN 4317596, referred to as the user guide for the
7900HT system throughout the rest of this document) for more
information.
A copy of the user guide is shipped with the 7900HT system.
Note:
An electronic copy is included on the Micro Fluidic Card Information
CD that comes with your Micro Fluidic Card order.
The Micro Fluidic Card allows you to use the 7900HT system for
profiling gene expression using the Comparative C
relative quantification. The card evaluates from one to eight cDNA
samples or controls generated from total RNA in a two-step RT-PCR
experiment.
Key Features
Following are the key features of the Applied Biosystems 7900HT
Micro Fluid Card:
• Assays-on-Demand
RT-PCR applications
• Flexible card format handles multiple sample/target
combinations
• Small-volume design minimizes sample and reagent
consumption
• Streamlined reaction set-up saves time and reduces labor-
intensive steps
Applied Biosystems Micro Fluidic Card Getting Started Guide
DRAFT
™
Gene Expression Products for all real-time
®
RISM
Method of
T
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