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Performing Real-Time Data Analysis
Summary
Where You Are in
the Procedure
You
Are Here
Applied Biosystems Micro Fluidic Card Getting Started Guide
Real-time is the term used to describe the category of sequence
detection runs in which the ABI P
Detection System is used to measure the fluorescence of a biological
sample during thermal cycling. Real-time experiments can be used to
achieve both qualitative and quantitative measurements.
Performing Reverse Transcription
(cDNA generation)
Loading, Centrifuging, Sealing and
Running the Micro Fluidic Cards
Performing Real-Time Data Analysis
(7900HT system and SDS software)
Interpreting Results
June 23, 2003 9:39 am, MFC_QSG_text.fm
Performing Real-Time Data Analysis
®
7900HT Sequence
RISM
Performing Real-Time Data
(7900HT system and SDS
1. Analyze the PCR sequence
detection run data.
2. Select analysis settings.
3. Eliminate outlying
amplification.
4. Export the analyzed data
for relative quantification.
DRAFT
Analysis
software)
29
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