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Absolute vs.
Relative
Quantification
30
June 23, 2003 9:39 am, MFC_QSG_text.fm
When calculating the results of your quantification assays, you can
use either relative or absolute quantification documents to perform
relative quantification analysis. The type of document depends on
whether you will use Applied Biosystems SDS 2.1 software or
custom software to perform RQ analysis.
Relative quantification describes the change in expression of the
target gene in a test sample relative to a calibrator sample. The
calibrator sample can be an untreated control or a sample at time zero
in a time-course study (Livak and Schmittgen, 2001). Relative
quantification provides accurate comparison between the initial level
of template in each sample.
For Micro Fluidic Card assays, relative quantification using the
comparative C
method is recommended.
T
The following documents provide more information about relative
quantification:
• Livak, K.J., and Schmittgen, T.D., 2001. Analysis of Relative
Gene Expression Data Using Real-Time Quantitative PCR and
–∆∆CT
the 2
Method. Methods 25:402-408.
®
• ABI P
7700 Sequence Detection System User Bulletin #2:
RISM
Relative Quantitation of Gene Expression (PN 4303859)
®
• ABI P
7900HT Sequence Detection Systems and SDS
RISM
Enterprise Database User Guide (PN 4317596)
• Relative Quantification Quick Start: ABI P
Sequence Detection System Quick Reference Card
(PN 4342505)
• RQ Manager Software User Guide (PN 4339753)
a. Contact your Applied Biosystems sales representative to obtain a copy of
this document.
Applied Biosystems Micro Fluidic Card Getting Started Guide
DRAFT
a
®
7900HT
RISM
a
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