Creating Micro Fluidic Card Documents - Applied Biosystems 7900HT Getting Started Manual

Creating Micro
Fluidic Card
Documents
20
June 23, 2003 9:39 am, MFC_QSG_text.fm
About Micro Fluidic Card Documents
To run a Micro Fluidic Card on the 7900HT system, you need the
®
ABI P 7900HT Sequence Detection System software (SDS 2.1
RISM
software) and a Micro Fluidic Card document (also called an SDS or
plate document). The 7900HT system uses the SDS document to
organize and store the fluorescence data gathered during the PCR
run.
Each SDS document (*.sds) stores information about a unique
consumable (in this case, a Micro Fluidic Card with a unique
barcode), including:
• Plate type
• Detector setup
• Target/sample configurations
• Thermal cycling parameters
• Data collection parameters
• Raw data gathered during the PCR process
There are two types of SDS documents for real-time PCR runs:
absolute quantification (AQ) and relative quantification (RQ). Use
the appropriate document type for your analysis, as described in
"Design Guidelines" on page
7900HT system contains more information about relative and
absolute quantification.
Setting up documents as RQ documents allows you to use
Applied Biosystems RQ Manager software for automated data
analysis. This software does not support analysis of AQ documents.
Applied Biosystems recommends that you use relative quantification
to analyze Micro Fluidic Cards. Chapter 6 of the user guide for the
7900HT system contains more information about setting up relative
quantification documents for the first time.
When creating SDS documents, make sure you create
IMPORTANT!
a document type (AQ or RQ) that is consistent with the type of
analysis for your study. You cannot readily convert RQ documents to
AQ documents, nor can you convert AQ documents to RQ
documents.
Applied Biosystems Micro Fluidic Card Getting Started Guide
DRAFT
12. Chapter 6 of the user guide for the