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Process
Overview
16
June 23, 2003 9:39 am, MFC_QSG_text.fm
The PCR process consists of the following procedures:
1. Preparing the sample-specific PCR mix.
See
page
17.
2. Loading the Micro Fluidic Cards.
See Chapter 4 of the user guide for the 7900HT system.
3. Centrifuging the Micro Fluidic Cards.
See Chapter 4 of the user guide for the 7900HT system and
page 19
for Micro Fluidic Card-specific guidelines on
centrifuge operation.
4. Sealing the Micro Fluidic Cards and trimming-off fill
reservoirs.
See Chapter 4 of the user guide for the 7900HT system.
IMPORTANT!
you go over the card in one direction, you cannot reverse the
process.
Sealing tape is not required for the card sealer. The
Note:
sealer makes grooves into the foil side of the card, sectioning
off the sample pocket from the main channel.
5. Creating Micro Fluidic Card Documents.
See
page
20.
6. Performing Micro Fluidic Card Runs.
See
page
26.
Applied Biosystems Micro Fluidic Card Getting Started Guide
DRAFT
The sealing process is unidirectional. Once
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