Guidelines For Loading Cards And Operating The Centrifuge - Applied Biosystems 7900HT Getting Started Manual

Guidelines for
Loading Cards
and Operating
the Centrifuge
Applied Biosystems Micro Fluidic Card Getting Started Guide
After the fill reservoirs of a Micro Fluidic Card have been loaded
with the sample-specific PCR mix, the centrifuge system distributes
the PCR mix into the wells of the card. Refer to Chapter 4 of the user
guide for the 7900HT system for information about loading the
centrifuge. Following are additional guidelines about loading Micro
Fluidic Cards:
• Install SDS 2.1 software on all instruments devoted to running
Micro Fluidic Cards.
• Load each Micro Fluidic Card with sample-specific PCR mix.
• Run Micro Fluidic Cards within 64 hours of loading them.
To ensure a high degree of reproducibility, Applied Biosystems
recommends scheduling your runs so that each Micro Fluidic
Card is run as soon as possible. After sealing, there is no
measurable well-to-well contamination for up to 64 hours.
Sorvall Legend and Heraeus centrifuges have either a touchpad
control (EASYSet models) or knob-operated control panel (QUIKSet
models). Both centrifuges use the same bucket type and adaptor. The
following table lists the operational parameters for Micro Fluidic
Cards.
Parameter
Up ramp rate
Down ramp rate
Rotational speed
Centrifugation time
Do not exceed 1200 rpm or accumulated centrifuge
IMPORTANT!
times of more than 3 min. Excessive centrifugation speeds and times
may deform Micro Fluidic Cards.
June 23, 2003 9:39 am, MFC_QSG_text.fm
Preparing and Running the Micro Fluidic Cards (PCR)
QUIKSet
3
N/A
1200 rpm
2 × 1 min
(0.01 on display)
DRAFT
EASYSet
9
9
1200 rpm
2 × 1 min
(0.01 on display)
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