How The Software Analyzes Protein Charge Variant Data - PerkinElmer LabChip GX User Manual

How the Software Analyzes Protein Charge
Variant Data
Protein Charge Variant assays are only supported on LabChip GX II
instruments.
The LabChip GX Protein Charge Variant Assay Analysis consists of
the following steps:
1
2
3
4
5
V4.2
Raw data is read and stored by the system for each individual
well.
The data is filtered (see
resulting electropherograms of all wells are plotted.
A baseline is generated for the data using the settings on the
Peak Find tab on the
displayed on the electropherogram when Show Peak Baselines
is selected in the Graph View Properties
Peaks are identified (see
page 108) for all wells and are tabulated by migration time. The
settings of the peak find algorithm can be changed and the data
can be reanalyzed after the run has finished. (See
a Data File" on page
for all or only certain wells.
The % Relative Amount for each peak is calculated as a percent
of total area.
LabChip GX User Manual
"Data Filtering" on page
Assay Analysis Window. This baseline is
"Understanding Peak Finding" on
95.) The peak find settings can be changed
Data Analysis
307) and the
(see page 164).
"Reanalyzing
PerkinElmer
57