How The Software Analyzes Dna Data - PerkinElmer LabChip GX User Manual

How the Software Analyzes DNA Data

The LabChip GX
concentration of nucleic acid fragments. Results for each well are
calculated after all data for that well has been read.
The data analysis process for DNA assays consists of the following
steps:
1
2
3
4
V4.2
DNA Assay Analysis
Raw data is read and stored by the system for each individual
well.
The data is filtered (see
resulting electropherograms of all wells are plotted.
Peaks are identified (see
page 108) for all wells and are tabulated by migration time. The
settings of the peak find algorithm can be changed and the data
can be reanalyzed after the run has finished. (See
a Data File" on page
for all wells or only for specific wells.
A sizing ladder (see
fragments of different known sizes, is run first from the ladder
vial. The concentrations and sizes of the individual base pairs in
the ladder can be changed on the
Figure 8. Ladder Graph - DNA
LabChip GX User Manual
calculates the size and
"Data Filtering" on page
"Understanding Peak Finding" on
95.) The peak find settings can be changed
Figure 8), which is a mixture of DNA
Analysis
Data Analysis 43
307) and the
"Reanalyzing
Tab.
PerkinElmer