Advanced Tab - PerkinElmer LabChip GX User Manual

Advanced Tab

Use the Advanced tab on the
Quantification, Peak Size Calibration point, Concentration Size
Correction, Peak Integration Mode, and Marker Find settings.
Quantification
Determines whether the upper marker, lower marker, or a sample
peak is used for quantitation. Protein, RNA, and gDNA assays use
the lower marker. DNA assays use the upper marker. DNA and RNA
settings cannot be changed. For Protein and gDNA assays,
selecting Sample Peak uses the Peak Size and Concentration of
the User Standard instead of the default ladder settings. Not
available for Glycan or Protein Charge Variant assays. (Protein,
Glycan, and Protein Charge Variant assays are only supported on
LabChip GX II instruments.)
Peak Size Calibration Point
Specifies which part of the peak is used as the size calibration
point.
•
•
Concentration Size Correction (Protein assays only)
Protein concentration is size-corrected to account for differences in
the amount of protein injected into the separation channel based on
its size. The correction is obtained using the formula:
•
The default values for the Concentration Size Correction were
determined empirically by measuring a wide range of proteins of
known size and concentration. This correction can be bypassed by
setting Amplitude = 1.0, Power = 0.0.
V4.2
Figure 81. Assay Analysis Window - Advanced Tab
Leading Edge - If selected, uses the start of the peak. (May be
better when sample peaks are broader than ladder peaks.)
Center - If selected, uses the top of the peak. (May be better
when the leading edge is not well-defined.)
Conc(Size Corrected) = Conc(Time Corrected Area) * Amplitude
* (Size)^Power
LabChip GX User Manual
Software Reference 205
Assay Analysis Window to select the
PerkinElmer