PerkinElmer LabChip GX Touch User Manual
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PerkinElmer LabChip GX Touch User Manual

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LabChip
GX Touch/GXII Touch
User Manual
P/N CLS151164 Rev. B
Publication Date: October 12, 2018

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Summary of Contents for PerkinElmer LabChip GX Touch

  • Page 1  LabChip GX Touch/GXII Touch User Manual P/N CLS151164 Rev. B Publication Date: October 12, 2018...

  • Page 2: Preface

    PerkinElmer reserves the right to revise this publication and to make changes from time to time in the content hereof without obligation of PerkinElmer to notify any person of such revision or changes. Further, PerkinElmer may make modifications to the product described in this manual at any time without any obligation to notify any person of such modifications.
  • Page 3 Software version and firmware version (found by touching the Info View button in the lower right corner of the LabChip GX Touch software) • If applicable, the error number shown in the LabChip GX Touch software or in the log file. Product Service and Customer Support Plans PerkinElmer offers a full range of services to ensure your success.
  • Page 4 REMARQUE Tout changement ou modification apporté à cet instrument non expressément approuvé par l’entité responsable de la conformité peut annuler l’autorisation d’opérer l’appareil accordée à l’utilisateur. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 5 Risque de lésion oculaire; porter des lunettes de sécurité. Risk of fire. (ISO 3864) Risque d’incendie. Risk of poison. (ISO 3864) Risque d’empoisonnement. Risk of explosion. (ISO 3864) Risque d’explosion. Hazardous fumes. Émanations dangereuses. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 6 Signifie que l’appareil a réussi les tests de sécurité pour la mise à la terre, le courant transitoire de ligne d’alimentation et la perte de courant. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 7 Green Safe condition or information Jaune Attention, danger potentiel Les étiquettes Rouge Arrêter de l’appareil Bleu Intervention obligatoire sont codées Vert Condition sûre ou informations de sécurité couleur: P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 8: Instrument Safety

    Instrument Safety Instrument Safety The following safety information about the LabChip GX Touch/GXII Touch is included in this documentation. Read and review all safety information before operating the LabChip GX Touch/GXII Touch. • Required Training • Chemical Safety on page 9 •...

  • Page 9: Chemical Safety

    Instrument Safety Chemical Safety WARNING Some chemicals used with the LabChip GX Touch/GXII Touch are potentially hazardous and can cause illness. • Read and understand the material safety data sheet (MSDS) provided by the chemical manufacturer before you store, handle, or work with any chemical or hazardous material.

  • Page 10: Laser Safety

    Laser Safety WARNING BRIGHT LIGHT HAZARD. LabChip GX /GXII Touch Instruments contain Class 3B laser diodes. The LabChip GX Touch/GXII Touch are classified as a Class 1 device because the lasers are appropriately enclosed (embedded) and indicated with Warning labels.

  • Page 11: Electrical Safety

    Instrument Safety Electrical Safety The LabChip GX Touch/GXII Touch is powered by a UL/CSA/VDE approved 100-240 VAC, 50/60 Hz input, 5, 15, 24 VDC output power supply. Additionally, the LabChip GX Touch/GXII Touch High Voltage circuitry is current-limited to non-hazardous levels. Users...
  • Page 12 EN 60 320/IEC 320 Standard Sheet C13 connector for mating with appliance inlet on the system. Fuses The LabChip GX Touch/GXII Touch instruments contain two fuses. Changing the Fuses on page 142 for fuse replacement instructions. Contact PerkinElmer Technical Support (see...

  • Page 13: Mechanical Safety

    Instrument Safety Mechanical Safety The LabChip GX Touch/GXII Touch instruments have a three axis robot that moves quickly and can be a pinch hazard. Keep the front door of the instrument closed and keep hands away from the plate loading position when the robot is moving. Keep hands away from the robot when not actively placing microplates in the instrument or changing the ladder and buffer tubes.

  • Page 14: Table Of Contents

    Assay User and Quick Guides ................19 Principles of Operation ..................20 Preparing the Instrument to Run Samples ............23 Opening the LabChip GX Touch Software ............24 Purging the Pressure Lines ................24 Inserting the Chip ....................25 Calibrating the Instrument to Run Protein Clear HR Assays ......26 Priming the Chip Before the Run ...............
  • Page 15 Software Reference ....................66 CDR/Database Server Window ................. 67 Change Assay Folder Window................68 Change Password Window................69 LabChip GX Touch Main Window ..............70 Navigation Bar....................71 Home Window ....................72 Login Window....................74 Prime and Calibrate Window ................76 Prime Window ....................
  • Page 16 Sample Names Tab ................... 120 Analysis Settings Tab ................122 Wash Window ....................124 LabChip GX Touch/GXII Touch Instrument Description ........125 Front View ....................... 126 Rear Connectors ..................... 128 Side Connectors....................129 Optics ......................130 Chip Pressure System ..................130 Barcode Reader ....................
  • Page 17 Cannot Connect to Remote CDR Server ............ 164 Zipping the Log Files ..................165 Glossary of Terms ....................166 PerkinElmer Product Warranty ................178 PerkinElmer Software License Agreement ............180 Index ........................184 P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 18: Introduction

    • Principles of Operation on page 20 Usage LabChip GX Touch software is for use with LabChip GX/GXII and GX Touch/GXII Touch instruments. LabChip GX/GXII and GX Touch/GXII Touch instruments are for research use only and not for use in diagnostic procedures.

  • Page 19: Assay User And Quick Guides

    Assay User Guides provide information about a specific assay. Instructions for preparing the chip, the plate, the ladder tube, and the buffer tube are included in the LabChip GX Touch/GXII Touch Assay User Guide for the specific assay that you are running.

  • Page 20: Principles Of Operation

    Introduction Principles of Operation The LabChip GX Touch assays are based on traditional gel electrophoresis principles that have been transferred to a chip format. The chip format dramatically reduces separation time and provides automated sizing and quantitation information in a digital format.
  • Page 21 The data can be viewed in a gel-like format on the Gel Tab to achieve the appearance of a slab gel. (The colors of the gel can be changed.) Figure 3. Gel View P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 22 For Protein Charge Variant assays, analytes are separated based on their net charges, with molecules with a higher net charge migrating faster than those with a lower net charge. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 23: Preparing The Instrument To Run Samples

    Touch Assay User Guide for the specific assay that you are preparing to run. For instructions on preparing the chip and plate, see the LabChip GX Touch/GXII Touch Assay Quick Guide for the specific assay. The current version of the Assay User Guides and Quick Guides are available on the PerkinElmer web site at: www.perkinelmer.com.

  • Page 24: Opening The Labchip Gx Touch Software

    Type a valid LabChip GX Touch user name and password into the text boxes and touch the Logon button. LabChip GX Touch user names are created in the LabChip GX Reviewer software. (For instructions on creating LabChip GX Touch user names, see the LabChip GX Reviewer User Manual.)

  • Page 25: Inserting The Chip

    Protein Clear HR assays (see page 26). If any other chip is inserted into the instrument, the chip can be primed while preparing the first plate (see page 27). P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 26: Calibrating The Instrument To Run Protein Clear Hr Assays

    To calibrate the instrument to run Protein Clear HR assays: Insert the Protein Clear HR chip (see page 25) OR Open the LabChip GX Touch software (see page 24) with the chip already inserted into the instrument. Touch the Prime and Calibrate button on the Home Window.

  • Page 27: Priming The Chip Before The Run

    If desired, select the Run Test Ladder after Prime check box to run one ladder after the prime is complete. Touch the Prime button. The Prime Window displays the time left until the prime is complete. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 28: Placing The Barcode On The Plate

    (portrait) end of the microplate, closest to well A1. If the barcode is not positioned properly, the barcode reader will not be able to read the barcode. Figure 4. Barcode Label Position P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 29: Defining A New Plate Type

    Use an old, expired chip with a good sipper. • On the Plate Editor Tab, select the new plate name and touch the Verify Plate button. • Follow the onscreen instructions. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 30 If all holes are too close to the edge (<0.5mm) in the same direction, adjust the X-Margin or Y-Margin values on the Add Plate window. Select the new plate name on the Setup Run Tab when starting the run. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 31: Loading The Plate, Ladder Tube, And Buffer Tube

    Place the sample plate onto the plate carrier. Place the Ladder tube and Buffer tube into the plate carrier. Touch the Load Plate button. The plate carrier retracts into the instrument. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 32: Running An Assay

    41). Store the chip according to the instructions in the Assay User Guide. If necessary, wash the chip (see page 42) immediately before running the chip again. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 33: Defining The Run Parameters

    • To select or clear all wells in a quadrant, touch the 1, 2, 3, or 4 button. • To select or clear single wells, touch the well. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 34 Touch the Browse button, select the desired location for the data files in the Browse for Folder window, and touch the OK button. To restore the default path, touch the Default button. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 35 Open button. The path and name of the file displays in the text box. (See Sample Names File on page 177 for more information.) P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 36 47). • Edit the sample names (see page 60). • Edit the analysis settings (see page 38). • View the raw data from the run (see page P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 37: Monitoring The Run

    Running an Assay Monitoring the Run After a run is started in the LabChip GX Touch software: The priming and warming steps are performed. • The priming step fills the channels of the chip with reagent. (Only performed if the chip holder has been opened since the last run and the chip has not been primed.)

  • Page 38: Editing The Analysis Settings During A Run

    41) as necessary. Editing the Analysis Settings during a Run Specific analysis settings can be adjusted in the LabChip GX Touch software while a plate is running. If Auto Export is selected on the Setup Run Tab, the Analysis Settings cannot be changed unless the Defer Export to Run Completion check box is selected.

  • Page 39: Stopping A Run Before The Run Is Complete

    Figure 7. Stopped by User Data for any completed wells displays in the Views Window. To continue to read wells from an aborted run, see Continuing a Stopped Run. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 40: Continuing A Stopped Run

    Tab, touch the Start button to begin. The new run starts with the well that was not completed in the previous run. A separate data file is created for the current run. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 41: Removing The Plate, Buffer Tube, And Ladder Tube

    Lift the chip door up. Remove the chip from the chip holder. Close the chip door and press down to latch the door. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 42: Washing The Chip

    Touch the Home button on the Navigation Bar. Touch the Wash button on the Home Window. When the wash is complete, follow the instructions in the Assay User Guide to run the chip. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 43: Exporting Data

    • Exporting Data in AIA Format for Use in Third-Party Analysis Software on page 47 • Exporting a Data File after a Run is Complete on page 48 P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 44: Export Examples

    In addition to exporting time and value information, you can choose to export Size information. This information is determined based on aligned data and is used to correlate the peaks from one row to another. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 45 The Height selector on the Export tab determines the height, in pixels, of the exported graphic. Figure 9 shows all gels exported to one image file. Figure 9. Exported Gels P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 46: Selecting The Auto Export Settings

    Start the Run (see Running an Assay on page 32). After the run has started, change the exported properties in the Well Table or Peak Table if desired (see page 47). P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 47: Selecting The Exported Properties In The Well Table Or Peak Table

    Select the Raw Data check box under Export Selections. Select the AIA Format check box under Raw Data Options. Touch Save to save the Export settings. Start the Run (see Running an Assay on page 32). P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 48: Exporting A Data File After A Run Is Complete

    Views Window. If Peak Table or Well Table are selected for export, the exported table columns are selected in the Export Setup Tab on the Tools Window. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 49: Viewing The Data

    Views Window can display either analyzed or raw data. This section includes the following procedures for viewing data in the LabChip GX Touch software during a run: • Selecting the Well Data to View on page 49 •...

  • Page 50: Viewing Data In The Egram/Gel Tab

    To change the graph display options, select the desired options in the Graph Settings on the EGram Tab. To change the gel display options, select the desired options in Gel Settings on the Tab. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 51 RFU value specified. Figure 11 shows the electropherograms of two wells in the same graph, offset by 50 RFU. Figure 11. Overlay Offset P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 52: Viewing Graphs In The Egram Tab

    Dragging horizontally pans the view of the gels. • You can continue zooming in until you reach the maximum magnification (the graph will not zoom in any closer). P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 53: Viewing A Data File After A Run Is Complete

    Review Plate window to clear the current plate data. Select the desired data file to open in the Select a Data File window and then touch the Open button. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 54: Creating A Run File

    . The Save Run Parameters to File window opens. Type the desired name for the run file, select the file location, and touch the Save button to save the run file. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 55: Using Sample Names Files

    Sample Names files are also used to import sample names into a data file as the data file is created. This section includes general instructions for using Sample Names files in the LabChip GX Touch software: • Creating a Sample Names File on page 56 •...

  • Page 56: Creating A Sample Names File

    Using Sample Names Files Creating a Sample Names File Sample Names Files created in the LabChip GX Touch software can be used to specify the desired sample names for each well in a plate. The Sip order can also be specified to match the order of wells in the Sample Names file.

  • Page 57: Selecting Wells Using A Sample Names File

    Sample Names file. When using a Sample Names file to select the wells, only wells with an entry in the Sample Names file are selected for sampling. Use the LabChip GX Touch software, LabChip GX Reviewer software, Excel, or a LIMS to create the Sample Names file.
  • Page 58 Select the Sample Names file check box. Touch the Browse button next to the Sample Names text box, select the Sample Names file (*.csv), and touch the Open button. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 59: Using A Barcode To Specify The Sample Names File

    Selection check box is automatically selected when the Sample Names file contains the string %barcode%. In the Select Wells tab, select the desired Sipping Order options: Column-wise and/or File Order. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 60: Editing Sample Names During A Run

    Add comments to the Comment fields as desired. Touch the Apply button to save the changes. You must touch Apply before the well read is complete. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 61: Saving Data Files

    CDR when the network connection is restored. Organizing, Retrieving, and Backing Up Data Files As you work in the LabChip GX Touch software, it is a good practice to organize the LabChip GX Touch data files. •...

  • Page 62: Labchip Gxp Option

    LabChip GxP Option LabChip GxP Option A LabChip GxP option is available for the LabChip GX Touch software. This option contains built-in technical controls and features specifically designed to provide compatibility with 21 CFR Part 11 requirements. Users are responsible for establishing...

  • Page 63: Locking And Unlocking The Software

    Locking and Unlocking the Software The LabChip GX Touch software with the LabChip GxP option installed allows you to lock the LabChip GX Touch software. The software also locks after it has been left unattended for an amount of time specified by the LabChip GX Touch Administrator.

  • Page 64: User Accounts

    User Accounts Access to the LabChip GX Touch software is controlled by user accounts when the LabChip GxP option is installed. Each user must sign into the LabChip GX Touch software from the...

  • Page 65: Central Data Repository (Cdr)

    The LabChip GX Reviewer software enables you to create new folders, rename existing folder, and delete empty folders. CDR Security Suggestions To ensure proper security of data files, the LabChip GX Touch Administrator should: Change the administrator password. Make sure to keep a copy of the password in a safe place.

  • Page 66: Software Reference

    Software Reference Software Reference This section describes the windows in the LabChip GX Touch software. Each topic describes the options and buttons on the window, and how to open the window. • CDR/Database Server Window on page 67 • Change Assay Folder Window on page 68 •...

  • Page 67: Cdr/Database Server Window

    CDR is not installed yet, an error message displays. Cancel button - Closes the window without connecting to the remote CDR Server. If the LabChip GX Touch software is not connected to the CDR, assays cannot be run until the CDR is connected.

  • Page 68: Change Assay Folder Window

    New Folder button - Enables you to create new assay folders. Rename button - Enables you to rename the current assay folder. Use the LabChip GX Reviewer software to create new assay folders. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 69: Change Password Window

    Use the Change Password Window to change the password for the current user. This window is only available if the LabChip GxP option is installed with the LabChip GX Touch software. To open the Change Password Window, touch the User button on Home Window and then touch the Change Password button.

  • Page 70: Labchip Gx Touch Main Window

    Software Reference LabChip GX Touch Main Window Use the LabChip GX Touch main window to access the Navigation Home Window. The LabChip GX Touch Main Window opens when the software is opened. Figure 18. LabChip GX Touch Main Window P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 71: Navigation Bar

    After a new run is started or the software is closed, data from a completed run is no longer available in the LabChip GX Touch software. Use the LabChip GX Reviewer software to view or analyze data from previous runs.

  • Page 72: Home Window

    Protein Clear HR chips). Prime and Calibrate - Opens the Prime and Calibrate Window calibrate the instrument to run Protein Clear HR assays (only for Protein Clear HR chips). P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 73 Running - The GX Touch is running an assay. • Warming - The GX Touch is warming a chip. • Washing - The GX Touch is washing a chip. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 74: Login Window

    Touch online help, and the Log Zipper function. Login Window Use the Login Window to log in to the LabChip GX Touch software, lock the software, switch users, or change passwords when the LabChip GxP option is installed. The LabChip GX Touch software will not start until a valid user name and password are entered.
  • Page 75 Account Policies specify additional password requirements. NOTE The Switch User, Change Password, and Lock buttons are only available if a user is already logged into the LabChip GX Touch software. Switch User button - Enables the User Name and Password text boxes to enable a new user to log in.

  • Page 76: Prime And Calibrate Window

    Select Plate Type drop-down list - Select the desired plate type to be used during the run. Prime and Calibrate button - Starts the calibration process. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 77: Prime Window

    Run Test Ladder after Prime check box - If selected, runs one ladder well after priming is complete. Prime button - Starts priming the chip and displays the status of the priming step. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 78: Run Window

    Run Window, touch the Run button on the Home Window. The Run Window contains the following tabs: • Select Wells Tab • Setup Run Tab • Start Run Tab P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 79: Select Wells Tab

    Import Run File button - Opens the Import Run Parameters from XML File window to select a run file to import. See Creating a Run File on page 54 for details on run files. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 80 Random Select% is set to 50%, five wells, selected at random, will be sampled during the run. Next button - Opens the Setup Run Tab. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 81: Setup Run Tab

    If selected, the priming process is not repeated. Skip Warm check box - Only displays if a ProteinEXact chip has been warmed before the run. If selected, the warming process is not repeated. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 82 Setup Run Tab (Continued) Read Plate Barcode check box - Only displays if the instrument is equipped with a barcode reader. If selected, the LabChip GX Touch reads the customer-applied barcode on the short (portrait) end of the plate. The barcode is saved in the data file and can be used as part of the data file name.
  • Page 83 (Optional) Computer Name check box - If selected, adds the name of the LabChip GX Touch computer to the data file name. Barcode check box - Only displays if the instrument is equipped with a barcode reader. If selected, the instrument reads the customer-supplied barcode on the short (portrait) edge of the microplate and includes the barcode in the data file name.

  • Page 84: Start Run Tab

    Window. Figure 25. Start Run Tab The Start Run Tab contains: Chip Status - Displays the Assay Type, Chip Reagent Expiration, Chip Expiration date, and Chip Life. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 85 (.xml). Start button - Runs the plate using the current settings. Status Window opens to view the status of the run or priming and calibration. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 86: Status Window

    Prime/Calibrate Status (Protein Clear HR and ProteinEXact chips only) - Displays the status of the current or just completed prime and calibration. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 87 Flashing green/orange - Well reading is in process. • Green - Well is selected for reading but not read yet. • Gray - Well is not selected to be read. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 88 EGram Tab in the Views Window. Gel - Displays the Gel of the well in progress during a run or prime/calibrate, or the last completed well. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 89: Tools Window

    The Tools window contains the following tabs: • Export Setup Tab • Diagnostics Tab • Optics Normalization Tab • Plate Editor Tab • Software IQ Tab • Factory Access Tab • Error Message Display P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 90: Export Setup Tab

    Figure 29. Setup Tab The following tabs display on top of the Export Setup tab: • Export Settings Tab • Peak Properties Tab • Well Properties Tab P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 91 (gel, egram, etc.), %COMMENT% = user comments. When a single file for the plate is exported, the %NAME% and %LABEL% fields are blank, and any leading, trailing or duplicate separators are removed. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 92 CSV Delimiter drop-down list. File Format for Gel and Electropherogram • Select the desired format for the exported image files. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 93 Exported list box - Lists the columns in the Peak Table that will be included in the exported Peak Table. Left and Right arrow buttons - Move the highlighted table column to the Not exported or exported list boxes. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 94 Exported list box - Lists the columns in the Well Table that will be included in the exported Well Table. Left and Right arrow buttons - Move the highlighted table column to the Not exported or exported list boxes. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 95: Diagnostics Tab

    Blue - The test is in progress. • Yellow - The test was skipped. • Green - The test passed. • Red - The test failed or was aborted. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 96 The bottom of the window displays the status, an overall progress bar for the entire set of selected tests and a separate test progress bar for the test that is currently running. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 97: Optics Normalization Tab

    Contrast - The contrast measured on the current instrument. New Correction Factor - The correction factor calculated from the instrument (Reference Contrast / Scan Results Contrast). P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 98: Plate Editor Tab

    Figure 34. Plate Editor Tab The following tabs display at the top of the Plate Editor tab: • Predefined Plates Tab - Displays PerkinElmer-defined plates. These plates are read-only and cannot be edited or deleted. • Custom Plates Tab - Displays all user-created plates. Plates can be added, deleted, and modified.
  • Page 99 Verify Plate button - Opens the Verify Plate window to perform a puncture test using the selected plate information. Follow the instructions in the Verify Plate window to test the settings for the selected plate. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 100: Software Iq Tab

    Software IQ Tab Use the Software IQ tab to perform the IQ test. The IQ test verifies proper installation of the LabChip GX Touch software and verifies no unauthorized changes have been made to the software. To open the Software IQ tab, touch the Views button on the Navigation Bar and then touch the Software IQ button.
  • Page 101 Figure 36. Run IQ tab The Run IQ tab runs the following tests: Application Information - Verifies that the LabChip GX Touch Registry Entries exist. If all expected registry entries are found, displays Passed. If any registry entries are missing or have been changed, displays Failed.

  • Page 102: Factory Access Tab

    Print button - Opens the Print window to print the detailed Software IQ report. Factory Access Tab Used by PerkinElmer Field Service Engineers. Only for use by qualified Service Engineers. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual...

  • Page 103: Error Message Display

    The software settings can also cause peaks to be undetected, which can cause errors. Error Messages on page 147 for a list of errors and tips on preventing or resolving errors. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 104: Unlock Application Window

    Touch software after it automatically locks or after the software is locked by the current user. The Unlock Application Window displays on top of the LabChip GX Touch software when the software is locked. (The automatic lock option is set in the LabChip GX Reviewer software.) The Unlock Application window only displays if...

  • Page 105: Views Window

    Gel Tab on page 113 • Run Info Tab on page 116 • EventLog Tab on page 119 • Sample Names Tab on page 120 • Analysis Settings Tab on page 122 P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 106: Select Wells To View Tab

    Tools Window. If Peak Table or Well Table are selected for export, the exported table columns are selected in the Export Setup Tab in the Tools Window. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 107 Red Exclamation Point in Well - An analysis error has occurred in the well, such as no lower marker detected. • Black Exclamation Point in Well - An analysis warning has occurred in the well. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 108: Egram/Gel Tab

    Graph Settings on the EGram Tab. Gel - Displays a gel of each well in the run. To change the gel view settings, use the Gel Settings on the Tab. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 109: Egram Tab

    Graph Settings on page 111. While a run or calibration is in progress, the Status Window displays the raw (unanalyzed) data as it is being read from the chip. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 110 Down button - Moves the selection one well below the current well. UnZoom button - Zooms out to the previous zoom level. Unzoom All button - Zooms out and returns to the standard view. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 111 • Lower drop-down list - Labels each peak in the graph with a peak property selected from the drop-down list. Default is None. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 112 The legend shows the color used for each well. • Overlay Offset text box- Offsets each of the graphs on the X axis, by the RFU value specified. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 113: Gel Tab

    Gel tab. The wells selected in the Gel tab are synchronized with the wells selected in the Select Wells to View Tab, Egram/Gel Tab, and EGram Tab. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 114 Tab, and EGram Tab. Gel Settings check box - If selected, displays the gel display options for the Gel tab. See Gel Settings for a description of each option. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 115 Foreground color. Lane Label Font button- Opens the Font window to change the font, style, and size of the labels in the gel headers. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 116: Run Info Tab

    Assay Name - The name of the assay that was run on the plate. Software Version - The version of LabChip GX Touch software used to run the plate. Firmware Version - The version of firmware on the instrument used to run the plate.
  • Page 117 Y Margin - The distance from the outer edge of the plate to the center of well A1 in the Y direction. Chip ID - The ID number of the Chip used to run the plate. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 118 Calibrated I6 - The current applied to electrode 6 on the chip during the separation portion of the sample processing. Calibrated V7 - The current applied to electrode 7 on the chip during the separation portion of the sample processing. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 119: Eventlog Tab

    Date - The date and time the event or error occurred. • Source - The system component that generated the event or error. • Event Code - The event/error ID number used by PerkinElmer to troubleshoot errors. • Description - Text describing the event or error. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 120: Sample Names Tab

    User comment for each well in the plate that is running, the plate that is open for review, or the imported Sample Names file. Well Label column - Displays the well label for each well. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 121 P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 122: Analysis Settings Tab

    Peak Start Time and the height at the Peak Apex must be greater than this value. • Width (sec) - Specifies the minimum width of a peak in seconds. Peaks narrower than this time are not detected. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 123 • Cancel button - Cancels changes to the window and displays the last saved values or the default values if no changes have been saved. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 124: Wash Window

    Figure 50. Wash Window The Wash Window displays the Chip status, Run status, and Instrument status during the wash. To stop the wash step, touch the Stop button. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 125: Labchip Gx Touch/Gxii Touch Instrument Description

    LabChip GX Touch/GXII Touch instruments. All of the components described in this section are the same for the GX and GXII Touch instruments. The LabChip GX Touch runs DNA and RNA assays. The LabChip GXII Touch runs DNA, RNA, Protein, Glycan, and Protein Charge Variant assays.

  • Page 126: Front View

    LabChip GX Touch/GXII Touch Instrument Description 126 Front View Figure 51 shows the front view of the LabChip GX Touch/GXII Touch and identifies the parts on the front of the instrument. Touch Screen Power Button Status Indicator Lights Front Door Figure 51.
  • Page 127 LabChip GX Touch/GXII Touch Instrument Description 127 Front View (Continued) Touch Displays the software to control the instrument. Touch the screen to Screen select the desired operations. Power Turns the instrument and touch screen on. Illuminated when the Button instrument is on. To turn the instrument off, select Start --> Shutdown on the Windows desktop to shut down the software.

  • Page 128: Rear Connectors

    LabChip GX Touch/GXII Touch Instrument Description 128 Rear Connectors The rear connector is used to connect the LabChip GX Touch/GXII Touch instrument to the power supply. Figure 52. Rear Connector The following connectors are located on the back of the instrument: AC Power Plug the power cord into this connector and a power outlet.

  • Page 129: Side Connectors

    LabChip GX Touch/GXII Touch Instrument Description 129 Side Connectors The side connectors are used to connect peripherals, such as a mouse, keyboard, or external monitor, to the LabChip GX Touch/GXII Touch instrument and to connect the instrument to a network.

  • Page 130: Optics

    (red excitation and red emission) for DNA, RNA, Protein and Glycan chips and assays. Red Laser The LabChip GX Touch/GXII Touch instrument includes a high intensity, long-life red diode laser to excite fluorescence on microfluidic chips. It is expected to have a lifetime of tens of thousands of operating hours.

  • Page 131: Dna, Rna, And Protein Chips

    DNA, RNA, and Protein Chips The figures below show the format and parts of the microfluidic chips used in the LabChip GX Touch/GXII Touch instruments to perform DNA, Protein, RNA, and Glycan sizing and quantitation. Figure 54. Top of Chip Figure 55.

  • Page 132: Chip Interface

    LabChip GX Touch/GXII Touch Instrument Description 132 Chip Interface The Chip Interface holds the chip, provides the voltage to the chip channels to load, inject, and separate the sample, provides pressure to prime the chip with separation gel, and provides vacuum to the chip wells to pull samples from the microplate into the chip.
  • Page 133 Supplies pressure (positive or negative) to prime the chip or move samples through the chip. Warning DO NOT use Test Chip B in the LabChip GX Touch/GXII Touch. Putting Test Chip B into the LabChip GX Touch/GXII Touch instrument will cause permanent damage to the instrument.

  • Page 134: Microplate Carrier

    LabChip GX Touch/GXII Touch Instrument Description 134 Microplate Carrier The LabChip GX Touch/GXII Touch instruments contain a stepper motor driven robot that moves the microplate to be accessed by the LabChip. Ladder Tube Locator Buffer Tube Spring Sample Well A1...

  • Page 135: Specifications

    LabChip GX Touch/GXII Touch Instrument Description 135 Specifications This section lists the technical specifications for the LabChip GX Touch/GXII Touch instruments. Technical specifications are subject to change without notice. General Size (H x W x D) 27” (69cm) x 20” (51cm) x 19” (49cm) Weight 56 lbs.
  • Page 136 LabChip GX Touch/GXII Touch Instrument Description 136 Chip Temperature Control Temperature Range Ambient + 5°C to 40°C (no active cooling) Accuracy ± 0.5°C Thermal limit switch 65°C - 70°C Fluorescence Detection Detection Wavelength Bandpass 670-725 nm Data rates 20, 30, 40, 50, 60, 80, 100, and 120 Hz...

  • Page 137: Maintenance And Service

    Running Software IQ on page 143 • Running Operational Qualification (OQ) on page 144 The LabChip GX Touch/GXII Assay User Guides and Quick Guides contain LabChip Kit Essential Practices and instructions for preparing reagents and plates. Make sure to follow the instructions to ensure the optimum performance of your instrument.

  • Page 138: Cleaning The Chip Interface

    Chip Interface Monthly Cleaning Remove the O-rings from the top plate of the chip interface on the LabChip GX Touch/GXII Touch instrument. Soak the O-rings in DI water for a few minutes. Clean the O-ring faces by rubbing between two fingers.

  • Page 139: Calibrating The Optics

    Maintenance and Service 139 Calibrating the Optics The LabChip GX Touch software provides an Optical Calibration function using Test Chip C to calculate a correction factor for each individual LabChip GX Touch instrument. The correction factor is applied to all samples run on the instrument to provide a common absolute fluorescence across different instruments.

  • Page 140: Cleaning The Instrument Electrodes

    • Clean the optical lens with an optical wipe and DI water only. Using other cleaning materials, or cleaning vigorously, can damage the lens. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 141: Cleaning Test Chip C

    Dry the chip completely with a gentle air stream from oil free compressed and filtered house air or compressed air cans. Do not shake can before use. Hold can at a distance from the chip to prevent condensation. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 142: Changing The Fuses

    Maintenance and Service 142 Changing the Fuses The LabChip GX Touch/GXII Touch instruments use two fuses located in the power entry module: ® (2) 3.15A Slo-Blo 250V, 5x20mm (P/N CLS138847) To order replacement fuses, contact PerkinElmer Technical Support. See Contact Us on page WARNING •...

  • Page 143: Running Software Iq

    Running Software IQ The Software IQ (Installation Qualification) verifies proper installation of the LabChip GX Touch software and verifies no unauthorized changes have been made to the software. The IQ can be run whenever required by your laboratory procedures.

  • Page 144: Running Operational Qualification (Oq)

    After all tests are complete, the test report is generated and saved to a file for review, printing, and documentation purposes. Diagnostics on page 152 for details on running the Diagnostics. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 145: Troubleshooting And Diagnostics

    Troubleshooting and Diagnostics 145 Troubleshooting and Diagnostics This section contains the following topics to help troubleshoot problems with the LabChip GX Touch software: • Searching for Events in the EventLog Tab on page 145 • Viewing Current Events in the EventLog Tab on page 146 •...

  • Page 146: Viewing Current Events In The Eventlog Tab

    Date - the date the event or error occurred. Source - The source of the event or error. Event Code - The event/error ID number used by PerkinElmer to troubleshoot problems. Description - A detailed description of the event or error that occurred.

  • Page 147: Error Messages

    EventLog Tab. The error or warning description describes the problem and either contains information on how to resolve the problem or directs you to call PerkinElmer Technical Support (see page For specific information about an error or warning message, touch one of the links below.

  • Page 148: Device Is Disconnected

    Possible Causes Recommended Actions 1. Instrument power loss. Verify the instrument power cord is plugged in. 2. Computer went into Press the Power button on the LabChip GX Touch/GXII hibernation. Touch instrument. See page 126. Restart the LabChip GX Touch software.

  • Page 149: Move Timeout

    1. Robot failure Check for an object blocking the robot motion and remove the blockage. Restart the instrument. If the problem is not resolved, contact PerkinElmer Technical Support (see page Pressure Leak Detected Error Message: “Pressure leak detected. Check and clean the O- rings at the chip interface.”...

  • Page 150: Maximum Samples Exceeded

    138). 2. Re-priming can sometimes clear chip clogs. Open the front door, close the door, and prime the chip. See the LabChip GX Touch/GXII Touch Assay User Guide for troubleshooting. 3. If the problem is not resolved, contact PerkinElmer Technical Support (see page P/N CLS151164 Rev.

  • Page 151: Current Leakage Check Failed

    Make sure the network cable is connected to the unplugged or not LabChip GX Touch (see page 129). connected to the network. Make sure the LabChip GX Touch is logged into the network. Make sure the computer where the CDR is installed is connected to the network.

  • Page 152: Diagnostics

    Troubleshooting and Diagnostics 152 Diagnostics The LabChip GX Touch software contains a set of diagnostic tests to verify proper installation and operation of the LabChip GX Touch/GXII Touch instrument. These tests are not dependent on assay chemistry. To open the...

  • Page 153: Running Operational Qualification (Oq)

    Do not use test chips from LabChip GX in the LabChip GX Touch instruments. WARNING DO NOT use Test Chip B in the LabChip GX Touch/GXII Touch. Putting Test Chip B into the LabChip GX Touch/GXII Touch instrument will cause permanent damage to the instrument.

  • Page 154: Description Of Instrument Diagnostic Tests

    Disk Space Check Available disk space below 4 GB. Risk of losing data as disk space is used. Free up space on local hard drive. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 155 Pressure sensor out of calibration. Sensor Test Contact PerkinElmer Technical Support (see page Sipping Pressure Chip Clog in pressure line. Contact Interface Clog Test PerkinElmer Technical Support (see page P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 156 Chip Heater Ramp Initial chip temperature not achieved in Test time. Temperature ramp time not within spec may indicate heater disconnected or burned out. Contact PerkinElmer Technical Support (see page P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 157 Plate Handler Test Test Chip A Required Description Potential Cause of Failure Plate Sensor Check Faulty sensor. Contact PerkinElmer Absence Technical Support (see page Plate Sensor Check Presence P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 158 Optics Noise Test If the Optics Noise is greater than the limit, calibrate the optics (see page 139). If the problem still occurs, contact PerkinElmer Technical Support (see page P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 159 Plate missing barcode. Place barcode on plate in correct position (see page 28). Barcode is not read correctly. Barcode reader disconnected or misaligned. Contact PerkinElmer Technical Support (see page P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 160: Calibration Failures

    VeriMAb sample in the plate with fresh VeriMAb. Retry calibration (see page 26). If the problem is not resolved, contact PerkinElmer Technical Support (see page P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.

  • Page 161: Protein Clear Hr Assay Marginal Calibration

    VeriMAb sample in the plate with fresh VeriMAb. • Retry calibration (see page 26). If the problem is not resolved, contact PerkinElmer Technical Support (see page P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual...

  • Page 162: Proteinexact Assay Failed Calibration

    32). AND/OR Calibrate the Optics (see page 139). If the problem is not resolved, contact PerkinElmer Technical Support (see page 2. Expected standard Perform the Removing Sipper Clogs procedure arrival time is outside describe in the ProteinEXact Assay User Manual.

  • Page 163: Software Problems

    After the computer restarts, open another file, and save it to verify the Save function is working properly. LabChip GX Touch software is corrupted. Reinstall the software. If the problem persists, contact PerkinElmer Technical Support (see page P/N CLS151164 Rev.

  • Page 164: Computer Software Lock-Ups

    Unlock Application Window. If this is not successful, or if the LabChip GxP option is not installed, try to exit and then restart the LabChip GX Touch software. If this is not successful, exit the LabChip GX Touch software using the Task Manager: (Access the Task Manager through the Control Panel.)

  • Page 165: Zipping The Log Files

    PerkinElmer Technical Support. To zip the LabChip GX Touch diagnostics logs: In the LabChip GX Touch software, touch the Information (i) button in the lower right corner of any window to open the InfoView window.

  • Page 166: Glossary Of Terms

    Optical Calibration • Peak Baseline • Peak Identification • Protein Assay Analysis • Protein Charge Variant Assay • RNA Assay Analysis • Sample Names File • Start Time P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 167 Assay File File used by the LabChip GX Touch software to specify assay and default analysis settings, such as Ladder and marker sizes and concentrations, peak find settings, expected peaks, and excluded peaks.

  • Page 168: Data Files

    Data points are determined by the data collection rate set in the assay properties. Select the Show Data Points option in the Graph Settings to displays the data points used to generate the graph. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 169 An electrical potential is applied across a capillary containing a sample in a fluid medium. Positive molecules migrate towards the cathode and negative molecules migrate towards the anode at different speeds depending on their electrophoretic mobility. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 170 Peak Files are created in the LabChip GX Reviewer software. See the LabChip GX Reviewer User Manual for details on creating the Expected Peak file. GXD Files The file extension for data files created in the LabChip GX Touch software. See Data Files.
  • Page 171 As the threshold is increased, more peak splitting occurs. Analysis settings can only be changed when reviewing LabChip GX Touch data in the LabChip GX Reviewer software. Lab-on-a-Chip The generic term for a microfluidic product, signifying a chemical process or material movement taking place on a microchip.
  • Page 172 Protein Charge Variant assays do not use ladders or markers. Log Files The LabChip GX Touch software log file displays in the EventLog Tab. The log file maintains a running record of all events that occur with the instrument while it is online with the software and records all events that occur in the software.
  • Page 173 Glossary of Terms 173 Optical Calibration The LabChip GX Touch/GXII Touch instrument reads fluorescence of a laser illuminated dye attached to compounds present in a sample. The absolute value of this fluorescence has little meaning as all computed quantizations, such as concentration, are derived from ratios of fluorescence to known standards found in the supplied ladder.
  • Page 174 Show Peak Baselines option in the Graph Settings. The area of each peak is determined by trapezoidal integration of the peak signal between peak boundaries and above the peak baseline. P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 175 The peak concentration is then calculated using the ladder peak areas and concentration for the ladder. Analysis settings can only be changed when reviewing LabChip GX Touch data in the LabChip GX Reviewer software. To quantitize the sample peak concentration based on a different standard, the new standard must be added into each sample well at a known concentration.
  • Page 176 Protein Charge Variant assays and data files do not include any ladder or marker parameters or options. Analysis settings can only be changed when reviewing LabChip GX Touch data in the LabChip GX Reviewer software. RNA Assay Analysis...
  • Page 177 GX Reviewer or LabChip GX Touch software. See the LabChip GX Reviewer User Manual for details on creating the sample names file in LabChip GX Reviewer. Sample Name that contain 96 wells or 384 wells can be created in the LabChip GX Touch software (see page 56).

  • Page 178: Perkinelmer Product Warranty

    PerkinElmer Product Warranty PerkinElmer, Inc. (“PerkinElmer”) provides a limited warranty to the original purchaser of each product (each, a “Warranty”), as set forth below. Please note that extended warranty and support plans are available for purchase, under separate agreement. Please contact PerkinElmer customer support for more information.
  • Page 179 • Customer must perform the appropriate level of revalidation required as a result of any maintenance or service provided. • For on-site services, Customer must ensure that the site where the product is located is safe for PerkinElmer personnel to enter, prior to PerkinElmer performing any services under this Warranty.

  • Page 180: Perkinelmer Software License Agreement

    (the “Licensee”) and PerkinElmer (which means (i) PerkinElmer, Inc., if the Licensee is located in the United States; (ii) the local PerkinElmer, Inc. sales subsidiary, if the Licensee is located outside the United States and in a country in which PerkinElmer, Inc. has a local sales subsidiary;...
  • Page 181 Software. Data collected will not include any Licensee data created with the Software. Licensee consents to such detection and collection of data, as well as its transmission and use. PerkinElmer also reserves the right to use a hardware lock device, license administration software, and/or a license authorization key to control access to the Software.
  • Page 182 Software. Termination is not an exclusive remedy. Limited Warranty. PerkinElmer's sole warranty with respect to the Software is that for a period of ninety (90) days from the date of receipt, the Software will substantially contain the functionality described in the published specifications, and when properly installed on a computer meeting the specifications set forth in, and operated in accordance with, the Documentation, will substantially perform in accordance therewith.
  • Page 183 Agreement. PerkinElmer shall not be in default hereunder by reason of any failure or delay in the performance of its obligations hereunder where such failure or delay is due to strikes, labor disputes, civil disturbances, riot, rebellion, invasion, epidemic, hostilities, war, terrorist...

  • Page 184: Index

    ... . 59 Contact PerkinElmer ....3 P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 185 ....122 specification ....135 P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 186 ....172 Microplate ....134 P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 187 ..... 29 inserting ....31 P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 188 ....59 Storage Temperature ... . 135 P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 189 Viewing Current Events in the EventLog ......146 P/N CLS151164 Rev. B LabChip GX Touch/GXII Touch User Manual PerkinElmer, Inc.
  • Page 190 PerkinElmer Health Sciences, Inc. 68 Elm Street Hopkinton, Massachusetts 01748 TEL 203-925-4602 FAX 203-944-4904 http://www.perkinelmer.com...

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