Changing The Peak Find Parameters - PerkinElmer LabChip GX User Manual

Changing the Peak Find Parameters

After data filtering, the peak find algorithm locates the peaks and
calculates the local peak baselines. The algorithm begins by finding
all the peaks above the noise threshold to determine the baseline,
after which any peaks below the noise threshold are rejected. A
local baseline is calculated for each peak to allow for baseline drift.
The following Peak Find parameters can be changed:
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To change the Peak Find parameters for all wells:
1
2
3
To change peak find settings for individual wells:
1
2
3
4
V4.2
Min Peak Height
Min Peak Width
Slope Threshold
Inflection Threshold
Start Time
End Time
Filter Width
Baseline Plateau
Select Analysis  Analysis Settings to open the
Analysis Window, and then click the
Change the parameters as necessary at the top of the window.
Click the OK button to save the setting, reanalyze the data, and
close the window.
Click the Apply button to apply the changes and reanalyze the
data, but keep the Assay Analysis window open.
Click the Apply Global button the apply the settings to all plates
in the workspace and reanalyze the data, but keep the Assay
Analysis window open.
Select Analysis  Analysis Settings to open the
Analysis Window, and then click the Peak Find Tab.
In the Well drop-down list, select the well number that you want
to change the settings for.
Change the settings at the bottom of the window under Well
Peak Find Settings to change the settings for the selected well.
Click the OK button to save the setting, reanalyze the data, and
close the window.
Click the Apply button to apply the changes and reanalyze the
data, but keep the Assay Analysis window open.
LabChip GX User Manual
Data Analysis
Assay
Peak Find Tab.
Assay
PerkinElmer
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