PerkinElmer LAMBDA 265 User Manual
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PerkinElmer LAMBDA 265 User Manual

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UV SPECTROMETERS
L
265
AMBDA
Users Guide

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Summary of Contents for PerkinElmer LAMBDA 265

  • Page 1 UV SPECTROMETERS AMBDA Users Guide...
  • Page 2 The information contained in this document is subject to change without notice. Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no warranty of any kind with regard to this document, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose.

  • Page 3: Table Of Contents

    Setting USB Serial Ports ................ 46 Operating Procedure ............... 51 Procedure .................... 52 Accessories ..................54 Accessories for the Lambda 265 ............. 55 Single Cell Holder ................55 Water Jacketed Single Cell Holder............ 56 Variable Pathlength Holder .............. 56 Advanced Transmission Holder ............57 Reflectance Module ................
  • Page 4 4 . Lambda 265 Users Guide...

  • Page 5: Introduction

    Introduction. 5 I ntroduction...

  • Page 6: Overview

    Overview The Lambda 265 instrument runs using UV Lab software. This manual provides instructions for installing and operating the Lambda 265 UV-Visible Spectrophotometer and UV Lab software. It also introduces and describes how to use basic features of the UV Lab software. For more detailed information on software features including kinetics, bio analysis, color analysis and MCA, refer to the UV Lab software user guide.

  • Page 7: About This Manual

    Information on installing and re-installing your instrument should you ever need to move your system is provided. Chapter 4 UV Lab Softw are I nstallation The complete UV Lab software installation procedures for the Lambda 265 is in this chapter. Chapter 5 I nterface Setup Interface procedures for the Lambda 265.

  • Page 8: Basic Theory

    8 . Lambda 265 Users Guide Basic Theory Electromagnetic radiation can pass through most organic and inorganic compounds. Generally, the UV range is from 190 to 380 nm and visible range is from 380-800 nm. Figure 1. Electromagnetic Radiation When continuous radiation passes through a sample, some is absorbed by the sample. An absorption spectrum can be obtained by monitoring the radiation that penetrates the sample and reaches a detector.

  • Page 9: Pda Uv-Visible Spectrophotometer

    Introduction. 9 Once a sample absorbs light energy, the intensity of incoming light P (the energy from the light source) reduces to P by losing some of its energy; therefore, we define the transmittance of a sample as follows: Figure 3. Sample Absorption As a result of absorption by a sample, transmittance is defined as;...

  • Page 10: Safety Summary

    10 . Lambda 265 Users Guide Safety Sum m ary The following safety symbols are used on this product. Warning Description Always refer to the system manual when working near locations at which the alert mark shown on the left is attached. If the operation, etc., is performed without heeding the advice in the System manual,...
  • Page 11 L'utilisateur ne doit pas essayer de réparer cet équipement. NE PAS tenter d'ouvrir le boîtier ou de démonter ses pièces internes. Seul un représentant du service PerkinElmer qui est conscient des risques d'incendie et d’électrocution devrait réparer cet équipement. Cet équipement contient des parties à haute tension présentant des risques de blessure grave ou d’électrocution au personnel non qualifié.
  • Page 12 12 . Lambda 265 Users Guide Warning Description For continued protection against risk of fire, replace only with same type and rating of fuse. Before changing the fuses, ALWAYS remove the power cord from the power-outlet and replace the blown fuses.

  • Page 13: Instrument Shipping Kit

    Introduction. 13 I nstrum ent Shipping K it The following table shows what is included in the Lambda 265 Spectrophotometer shipping kit. Item Name Qty. Main Lambda 265 Spectrophotometer Instrument Accessories Power Cord (US/EU/UK) 1/1/1 Interface Cable (USB) Fuse AC 250V (T2.0AL)

  • Page 14: Conventions Used In This Manual

    UPPERCASE text, for example ENTER or ALT, refers to keys on the PC keyboard. '+' is used to show that you have to press two keys at the same time, for example, ALT+F. All eight digit numbers are PerkinElmer part numbers unless stated otherwise. N otes, cautions and w arnings Three terms, in the following standard formats, are also used to highlight special circumstances and warnings.
  • Page 15 Introduction. 15 We use the term CAUTION to inform you about situations that could CAUTION result in serious damage to the instrument or other equipment. Details about these circumstances are in a box like this one. Caution (Achtung) Bedeutet, daß die genannte Anleitung genau befolgt werden muß, um einen Geräteschaden zu vermeiden.
  • Page 16 16 . Lambda 265 Users Guide We use the term WARNING to inform you about situations that could result in personal injury to yourself or other persons. Details about these circumstances are in a box like this one. WARNING Warning (Warnung) Bedeutet, daß...

  • Page 17: Contact Us

    If you are located within the U.S., call toll free 1-800-762-4000, 8 a.m. to 8 p.m. EST. Your order will be shipped promptly, usually within 24 hours. If you are located outside of the U.S., call your local PerkinElmer sales or service office.
  • Page 18 18 . Lambda 265 Users Guide...

  • Page 19: Safety Practices

    Introduction. 19 Safety P ractices...

  • Page 20: Overview

    This advice is intended to supplement, not supersede, the normal safety codes in the user's country. It is also a supplement to the PerkinElmer standard Safety and Health Policy. The information provided does not cover every safety procedure that should be practiced.

  • Page 21: Precautions

    The following precautions must be observed when using the Lambda 265: • Be sure that the voltage of the Lambda 265 corresponds to the voltage used in your laboratory. Les précautions suivantes doivent être respectées lors de l'utilisation du Lambda 265: Assurez-vous que la tension du Lambda 265 corresponde à...

  • Page 22: General Operating Conditions

    If any adjustment, maintenance and repair of the opened instrument is necessary, this must be done by a skilled person who is aware of the hazard involved. Whenever it is likely that the Lambda 265 is unsafe, make it inoperative. The Lambda 265 may be unsafe if it: •...

  • Page 23: Electrical Safety

    Safety Practices. 23 Electrical Safety The instrument has been designed to protect the operator from potential electrical hazards. This section describes some recommended electrical safety practices. Lethal voltages are present at certain areas w ithin the instrum ent. I nstallation and internal m aintenance of the instrum ent should only be perform ed by a P erk inElm er service engineer or sim ilarly authorized and trained person.
  • Page 24 If afterwards, the opened instrument must be operated for further adjustment, maintenance, or repair, this must only be done by a PerkinElmer Service engineer. Whenever it is possible that the instrument is no longer electrically safe for use, make •...

  • Page 25: Emc Compliance

    EM C Com pliance EC directive The Lambda 265 has been designed and tested to meet the requirements of the EC directive 2004/108/EC. The Lambda 265 complies with the EMC standard EN61326, (EMC standard for electrical equipment for measurement, control and laboratory use).

  • Page 26: Warning Labels

    WARNING Lorsque cette étiquette est attachée à un instrument, il est nécessaire de voir le manuel. See the following figure for the location of warning labels on the Lambda 265. Warning labels on the back of the instrument: Warning FUSE...

  • Page 27: Weee Instructions For Perkinelmer Products

    Customer Care telephone numbers select “Contact us” on the web page. Products from other manufacturers may also form a part of your PerkinElmer system. These other producers are directly responsible for the collection and processing of their own waste products under the terms of the WEEE Directive.

  • Page 28: Decontamination

    762-4000 or (+1) 203-925-4602, 8:30 a.m. - 7 p.m. EST and speak to Customer Support. In Canada, call toll free 800-561-4646 and speak to Customer Support. If you are located outside of the United States or Canada, please call your local PerkinElmer sales office for more information.

  • Page 29: Fuses

    Safety Practices. 29 Fuses There is risk of receiving a fatal electric shock if the fuses are replaced with the power cord connected. WARNING Il y a un risque d’électrocution si les fusibles sont remplacés tandis que le cordon d'alimentation est encore branché. The fuse is located at the rear of the instrument.
  • Page 30 30 . Product Title Hardware Guide...

  • Page 31: Lambda 265 Installation

    Lam bda 265 I nstallation...

  • Page 32: Preparing The Laboratory

    Pow er A grounded AC power supply must be used. The Lambda 265 accepts 100 to 240 VAC at 50 Hz or 60 Hz. I n order to protect the system from electrical noise and current surges, please connect the pow er cord to a GROUNDED OUTLET.
  • Page 33 Lambda 265 Installation . 33 Environm ental R equirem ents This system is for indoor use only. Requirement Description Indoor use Altitude up to 2000m (6,500 ft.) Pollution degree 2 Dimensions 340 mm(W) × 320 mm(D) × 115 mm(H) Weight 6.6 kg (14.6 lbs)

  • Page 34: Installation

    34 . Lambda 265 Users Guide I nstallation Carefully remove the instrument and all of its components from the shipping container. Verify that all of the components received match the packing list. Verify that all system components required for installation are present, this, must include: ...

  • Page 35: Uv Lab Software Installation

    UV Lab Software Installation UV Lab Softw are I nstallation...

  • Page 36: How To Install The Uv Lab Software

    36 . Lambda 265 Users Guide How to I nstall the UV Lab Softw are ® 1. Insert the UV Lab installation CD into the CD-ROM drive. InstallShield Wizard starts automatically. ® Wizard does not start automatically, select Computer → CD Drive.
  • Page 37 UV Lab Software Installation 4. Enter customer information, if necessary, and select Next. 5. The software will be installed in C:\ UV Lab as a destination folder. To install the software to a different folder, select Change and select the desired folder.
  • Page 38 38 . Lambda 265 Users Guide 6. Verify the current settings and select Install. 7. The following window shows the status of the UV Lab installation.
  • Page 39 UV Lab Software Installation 8. After the setup is completed successfully, select Finish. The UV Lab software icon will appear on the Desktop.
  • Page 40 40 . Lambda 265 Users Guide...

  • Page 41: Interface Setup

    I nterface Setup...
  • Page 42 42 . Lambda 265 Users Guide I nstalling the USB Drive Turn on the computer. And then insert the enclosed CD into the CD-ROM drive. Connect the USB between the computer and instrument. Click Computer → CD Drive → Open.
  • Page 43 Interface Setup . 43 5. Select LAMBDA 265 folder. 6. Select Windows XP_ 7_8, Vista, Server 2003 to 2008 win2000 R2 folder.
  • Page 44 44 . Lambda 265 Users Guide 7. Double Click USBCom-CDM_20828. 8. Select Extract.
  • Page 45 Interface Setup . 45 9. Select Next. 10. The following dialog box will appear. After installation is completed successfully, select Finish. 11. Change the USB serial Ports after finishing driver setup. See the following chapter.

  • Page 46: Setting Usb Serial Ports

    46 . Lambda 265 Users Guide Setting USB Serial Ports 1. Select Computer → Properties. 2. Select on Device Manager.
  • Page 47 Interface Setup . 47 3. Select the Ports (COM & LPT) to expand the listing. These are the devices currently connected to the COM ports. USB Serial Port (COMx) is visible when the driver installation is completed successfully. 4. Double click USB Serial Port (COMx) of the Ports (COM & LPT) section.
  • Page 48 48 . Lambda 265 Users Guide 5. Select on the Port Settings tab and select on the Advanced… button. 6. Change the parameter values as shown below. 1024 1024...
  • Page 49 7. Select OK after checking the changed parameter values. 8. Launch the UV Lab software. 9. If the Lambda 265 fails to communicate with the PC, change the COM Port Number as following procedure. 10. Open Advanced Setting for COMx window following procedure on page 469.
  • Page 50 50 . Lambda 265 Users Guide 12. Make sure that the changed COM Port Number is applied, and select OK. 13. Restart the computer after finishing driver setting.

  • Page 51: Operating Procedure

    Operating Procedure. 51 Operating P rocedure...

  • Page 52: Procedure

    52 Lambda 265 Users Guide Procedure Turn on the power switch. Start UV Lab software. Enter a Title, Sample Name and choose the Experiment Type. Select OK. Set method parameters. Select OK. Prepare blank and sample solutions. Fill a 10 mm pathlength cell to ~80% of its volume with the blank solution and place it into the cell holder.
  • Page 53 Operating Procedure . 53 The blank spectrum is displayed. Remove the cell from the cell holder. Fill a 10 mm pathlength cell to ~80% of its volume with the sample solution and place it into the cell holder. Click or select Run Sample in the Measure menu.

  • Page 54: Accessories

    Accessories...

  • Page 55: Accessories For The Lambda 265

    If you are located within the U.S., call toll free 1-800-762-4002, 8 a.m. to 8 p.m. EST. Your order will be shipped promptly, usually within 24 hours. If you are located outside of the U.S., call your local PerkinElmer sales or service office. Single Cell Holder Designed for a standard cell with a 10 mm pathlength.

  • Page 56: Water Jacketed Single Cell Holder

    56 . Lambda 265 Users Guide W ater Jack eted Single Cell Holder Use this accessory to control the temperature of a sample in a standard 10 mm rectangular cuvette. Optional stirring is available with a separately purchased stir controller. This accessory accommodates a temperature by circulator.

  • Page 57: Advanced Transmission Holder

    Accessories. 57 Advanced Transm ission Holder Use this accessory to measure the transmittance spectrum of various solid samples including films, glass, filters and optical components. Reflectance M odule Use this accessory to test coatings and semi-conductor wafers; especially effective for measuring film thickness.

  • Page 58: Position Multi Cell Holder

    58 . Lambda 265 Users Guide 8 P osition M ulti Cell Holder Use this accessory to measure eight samples automatically. This accessory is controlled by UV Lab software. W ater Jack eted 8 P osition M ulti Cell Holder Use this accessory to measure eight samples automatically.

  • Page 59: Nano Stick

    Accessories. 59 N ano Stick Use this accessory to measure micro-volume sample such as DNA, RNA and Protein. M agnetic Stirrer Assem bly Use this accessory to mix sample, this accessory is controlled by Control Unit. Control the speed of magnetic stirrer between 120 and 1200 rpm.

  • Page 60: Index

    60 . Lambda 265 Users Guide I ndex SB drive ............42 Accessories ............58 Laboratory Preparation ......... 32 Basic Theory ............8 Operating Conditions ..........22 Operating Procedure ..........54 Cleaning ............... 28 Cleaning the Instrument ........28 Computer Requirements ........33 Precautions ............
  • Page 61 Accessories. 61...

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