Liquid Line Connections - Waters Prep Series Installation And Maintenance Manual

Liquid line connections

The Waters Prep system Startup Kit includes a variety of tubing and fittings
for connecting to the fluid handling unit, injector, detector, and column. Refer
to the list in the Startup Kit for information on all included tubing and
fittings.
This section describes how to select and cut tubing and how to install fittings.
Considerations
• When you install or change tubing observe safe laboratory practices
regarding solvent handling. Refer to the Material Safety Data Sheets for
the physical and chemical properties of the solvents you use.
• Always stop the flow and vent line pressure with the purge valve before
loosening a fitting.
Caution:
This can cause tubing to separate from the fitting and spray
solvent.
• Minimize dead volume by using appropriate tubing in all parts of the
system. Cut tubing and install fittings as this section describes.
• Do not nick, kink, or sharply bend the tubing. This can restrict flow.
Repeated bending of tubing causes tubing to fail.
Tip:
• Subjecting the system to a wide temperature fluctuations can loosen
fittings. Before operating the system at a new temperature, allow the
system's temperature to equilibrate with the room temperature. When
you first start the system, check all connections for leaks, and retighten
fittings as needed.
Tubing selection
Various tubing sizes for readying the system for large-scale operation
accompany the Prep system. Use these guidelines when you select tubing to
connect the Waters 2487 Detector:
• For flow rates up to about 10 mL/min in a small-scale operation, connect
the column outlet to the reference side of the detector cell with
0.009-inch ID tubing.
This varies with solvent viscosity.
Tip:
Do not attempt to relieve pressure by loosening a fitting.
Assembling the system 2-21