Waters Prep Series Installation And Maintenance Manual page 102

Chromatography troubleshooting (Continued)
Symptom
Reproducibility errors
Baseline drift, rapid
3-18 Troubleshooting, Testing, and Maintenance
Possible cause
Solvent not properly
degassed or sparged
Incorrect chemistry
Incorrect integration
Injector problem
Column not
equilibrated
Detector not allowed to
warm up
Solvent contaminated
Solvent not properly
degassed (rapid or slow
drift)
Flow fluctuations (rapid
or slow drift
Incorrect wavelength
for solvent
Corrective action
Degas or sparge
solvent.
Check chemistry.
Incorrect integration.
Troubleshoot injector.
Equilibrate column.
Allow detector to warm
up until baseline is
stable (time varies
based on wavelength
and sensitivity).
Use fresh solvent.
Degas solvent.
Troubleshoot fluid
handling unit.
Use correct wavelength
and solvent (make sure
solvent does not have a
UV absorbance at the
wavelength used).